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Key Documents

05-594

Sigma-Aldrich

Anti-HSP90 Antibody

clone D7a, Upstate®, from mouse

Synonym(s):

Anti-EL52, Anti-HEL-S-65p, Anti-HSP86, Anti-HSP89A, Anti-HSP90A, Anti-HSP90N, Anti-HSPC1, Anti-HSPCA, Anti-HSPCAL1, Anti-HSPCAL4, Anti-HSPN, Anti-Hsp103, Anti-Hsp89, Anti-Hsp90, Anti-LAP-2, Anti-LAP2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

D7a, monoclonal

species reactivity

human, rabbit, pig, mouse, rat, bovine, chicken

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NM_001109785.2

UniProt accession no.

P11501

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... HSP90AA1(3320)

Specificity

Recognizes HSP90.

Immunogen

Full-length protein purified from chicken brain; clone D7a

Application

This Anti-HSP90 Antibody is validated for use in IP, WB for the detection of HSP90.

Quality

routinely evaluated by immunoblot on RIPA lysates from mouse 3T3/A31 fibroblasts or human A431 cells

Target description

90 kDa

Physical form

Format: Purified
Protein G Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH 7.4.

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Other Notes

Please refer to lot specific datasheet.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Interaction between the Rous sarcoma virus transforming protein and two cellular phosphoproteins: analysis of the turnover and distribution of this complex.
Brugge, J, et al.
Molecular and cellular biology, 3, 9-19 (1983)
Role of the nuclear receptor coactivator AIB1-Delta4 splice variant in the control of gene transcription.
Chien, CD; Kirilyuk, A; Li, JV; Zhang, W; Lahusen, T; Schmidt, MO; Oh, AS; Wellstein, A; Riegel, AT
The Journal of Biological Chemistry null
Miquéias Lopes-Pacheco et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 41(6), 2194-2210 (2017-04-28)
Premature degradation of mutated cystic fibrosis transmembrane conductance regulator (CFTR) protein causes cystic fibrosis (CF), the commonest Mendelian disease in Caucasians. Despite recent advances in precision medicines for CF patients, many CFTR mutants have not been characterized and the effects
The hsp90-based chaperone system: involvement in signal transduction from a variety of hormone and growth factor receptors.
Pratt, W B
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 217, 420-434 (1998)
Jason M Shapiro et al.
PloS one, 11(11), e0165227-e0165227 (2016-11-02)
We previously isolated AF20, a murine monoclonal antibody that recognizes a cell surface glycoprotein of approximately 90-110 kDa. The AF20 antigen is specifically expressed in human hepatoma and colon cancer cell lines, and thus could serve as a cancer biomarker.
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