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Supelco

Ascentis® Express 90 Å F5 (2 μm) HPLC Columns

L × I.D. 7.5 cm × 2.1 mm UHPLC Column

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About This Item

Kod UNSPSC:
41115700
NACRES:
SB.52

product name

Ascentis® Express F5, 2 μm UHPLC Column, 2 μm particle size, L × I.D. 7.5 cm × 2.1 mm

Materiały

stainless steel column

Poziom jakości

100

agency

suitable for USP L43

linia produktu

Ascentis®

Właściwości

endcapped

producent / nazwa handlowa

Ascentis®

opakowanie

1 ea of

Parametry

1000 bar max. pressure (14500 psi)
60 °C temp. range

metody

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

dł. × śr. wewn.

7.5 cm × 2.1 mm

powierzchnia

120 m2/g

zanieczyszczenia

<5 ppm metals

macierz

Fused-Core particle platform
superficially porous particle

grupa aktywna macierzy

PFP (pentafluorophenyl) phase

wielkość cząstki

2 μm

wielkość porów

90 Å pore size

pH robocze

2-8

Zastosowanie

food and beverages

metoda separacji

reversed phase

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Opis ogólny

Ascentis® Express 2.0 μm F5 is a high-speed, UHPLC column based on a Fused-Core® particle design. This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.4 μm thick porous shell and the small overall particle size of 2 μm. The bonded, endcapped, dimethylpentafluorophenylpropyl stationary phase of Ascentis® Express 2.0 μm F5 provides a stable, reversed phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming pi-pi and mildly steric interactions, F5 phases also retain compounds by polar interactions. Having polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis® Express 2.0 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Zastosowanie

UHPLC/MS Analysis of Mycotoxins on Ascentis® Express F5, 2 μm

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Informacje prawne

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
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Certyfikaty analizy (CoA)

Lot/Batch Number
USKJH001285
USKJH001284
USKJH001283
USKJH001281
USKJH001272

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Research shows that immunoassay techniques are not the best choice for the estimation of vitamin D in human blood samples. The main reasons are that some immunoassays are not able to distinguish between 25-OHD3 and 25-OHD2 vitamin D metabolites. Furthermore
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Alex D Batista et al.
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On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Imran Ali et al.
Journal of separation science, 35(23), 3235-3249 (2012-11-28)
New generation columns, i.e. packed with superficially porous silica particles are available as trade names with following manufacturers: Halo, Ascentis Express, Proshell 120, Kinetex, Accucore, Sunshell, and Nucleoshell. These provide ultra-fast HPLC separations for a variety of compounds with moderate
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