ZRB2740

Anti-CD43 Antibody, clone 1O6 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonim(y): Anti-MCT8/SLC16A2 Antibody, clone 1I9 ZooMAb^® Rabbit Monoclonal, CD43, GALGP, GPL115, Galactoglycoprotein, Leukocyte sialoglycoprotein, Leukosialin, Sialophorin
• Kod UNSPSC: 12352203
• NACRES: NA.43
• białko sprzężone: unconjugated
• application: FACS; ICC; IHC; WB; affinity binding assay
• klon: 1O6, recombinant monoclonal
• reaktywność gatunkowa: mouse, rat, human
• metody: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 200
• rekombinowane: expressed in HEK 293 cells
• białko sprzężone: unconjugated
• forma przeciwciała: purified antibody
• rodzaj przeciwciała: primary antibodies
• klon: 1O6, recombinant monoclonal
• opis: recombinant, expressed in HEK 293 cells
• linia produktu: ZooMAb^® learn more
• Formularz: lyophilized
• masa cząsteczkowa: calculated mol wt 40.32 kDa; observed mol wt ~80 kDa
• oczyszczone przez: using Protein A
• reaktywność gatunkowa: mouse, rat, human
• opakowanie: antibody small pack of 25
• charakterystyka ekologicznej alternatywy: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• rozszerzona walidacja: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• metody: affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG
• sekwencja epitopowa: Extracellular domain
• numer dostępu Protein ID: NP_001025459
• numer dostępu UniProt: P16150
• kategoria ekologicznej alternatywy: Aligned
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C
• informacje o genach: human ... SPN(6693)

Opis

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specyficzność

Clone 1O6 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects CD43. It targets an epitope within 14 amino acids from the extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the extracellular domain of human CD43.

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in EL4 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CD43 in EL4 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CD43 in lysates from Raji cells, Human spleen tissue, and Rat spleen tissue,and His-tagged recombinant fragment of Human CD43.

Affinity Binding Assay: vA representative lot of this antibody bound CD43 peptide with a KD of 2.7 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected CD43 in Human spleen tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CD43 in Raji cells.

Flow Cytometry Analysis: 0.1 µg from a representative lot detected CD43 in one million Raji cells and Human peripheral blood mononuclear cells (PBMC)..

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Opis wartości docelowych

Leukosialin (UniProt: P16150; also known as GPL115, Galactoglycoprotein, GALGP, Leukocyte sialoglycoprotein, Sialophorin, CD43) is encoded by the SPN (also known as CD43) gene (Gene ID: 6693) in human. CD43 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 20-253), a transmembrane domain (aa 254-276), and a cytoplasmic domain (aa 277-400). Its cleavage by cathepsin G is reported to release its extracellular domain and trigger its intramembrane proteolysis by -secretase thereby releasing the CD43 cytoplasmic tail chain (CD43-ct) that translocates to the nucleus. It has high content of sialic acid and O-linked carbohydrate structures CD43 expression is reported on surface of T-cells, neutrophils, plasma cells, and myelomas. Its expression on B-cells is an immunophenotypic feature suggestive of malignancy. CD43 regulates multiple funct

Postać fizyczna

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstytucja

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informacje prawne

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable