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ZRB1539

Sigma-Aldrich

Anti-Aquaporin 5 Antibody, clone 3H17 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonim(y):

AQP-5

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

rekombinowane

expressed in HEK 293 cells

białko sprzężone

unconjugated

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

3H17, recombinant monoclonal

opis

recombinant, expressed in HEK 293 cells

linia produktu

ZooMAb® learn more

Postać

lyophilized

masa cząsteczkowa

calculated mol wt 28.29 kDa
observed mol wt ~28 kDa

oczyszczone przez

using Protein A

reaktywność gatunkowa

human, mouse, rat

reaktywność gatunkowa (przewidywana na podstawie homologii)

canine, monkey, feline, bovine

opakowanie

antibody small pack of 25 μL

charakterystyka ekologicznej alternatywy

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

rozszerzona walidacja

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

metody

affinity binding assay: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

izotyp

IgG

sekwencja epitopowa

C-terminal

numer dostępu Protein ID

numer dostępu UniProt

kategoria ekologicznej alternatywy

Warunki transportu

ambient

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specyficzność

Clone 3H17 is a Rabbit recombinant monoclonal antibody that specifically detects Aquaporin-5 in multiple species. It targets an epitope within 25 amino acids from the C-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal, cytoplasmic domain of human Aquaporin 5.

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Aquaporin 5 in Jurkat cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Aquaporin 5 in lysates from MCF-7 and Raw264.7 cells and Mouse salivary gland tissue.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Aquaporin 5 (AQP5) in MCF-7 cell line.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Aquaporin 5 (AQP5) in human salivary gland tissue sections.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Aquaporin 5 (AQP5) in human salivary gland tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Aquaporin 5 with a KD of 1.7 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Opis wartości docelowych

Aquaporin-5 (UniProt: P55064; also known as AQP-5) is encoded by the AQP5 gene (Gene ID: 362) in human. Aquaporins, a highly conserved group of membrane proteins that are involved in the bidirectional transfer of osmotically driven water and small solutes across cell membranes. They contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA). Aquaporin-5 is a homotetrameric, multi-pass membrane glycoprotein that contains six cytoplasmic domains, six continuous transmembrane domains, two discontinuous transmembrane domains, and four extracellular domains. Its amino and carboxyl termini located in the cytoplasm. It is detected in skin eccrine sweat glands, digestive tract, renal cortex, and vaginal epithelial cells. In the respiratory system it is expressed in airways at the apical membrane of columnar epithelial cells and at the apical membrane of serous acinar cells of sub-mucosal glands. In lungs its expression at the apical membrane of type I pneumocytes. It plays an important role in fluid secretion in salivary glands and is shown to be essential for transient receptor potential cation channel subfamily V member 4) (TRPV4) activation by hypotonicity. Together with TRPV4 it controls regulatory volume decrease in salivary epithelial cells. Mutations in AQP5 gene have been linked to the development of palmoplantar keratoderma, a disease that is characterized by an abnormal thickening of the skin on the palms of the hands and soles of feet. Overexpression of Aquaporin-5 has also been observed in several human cancers and knockdown of AQP5 leads to partially reduced cell cycle progression from G1 to S phase and apoptosis. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Direito, I., et al. (2016). Cell. Mol. Life Sci. 73(8); 1623-1640).

Postać fizyczna

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstytucja

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informacje prawne

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

13 - Non Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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