Przejdź do zawartości
Merck
Wszystkie zdjęcia(13)

Key Documents

ZRB1506

Sigma-Aldrich

Anti-Substance P Antibody, clone 1K3 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352203
NACRES:
NA.41

pochodzenie biologiczne

rabbit

Poziom jakości

rekombinowane

expressed in HEK 293 cells

białko sprzężone

unconjugated

forma przeciwciała

purified antibody

rodzaj przeciwciała

primary antibodies

klon

1K3, recombinant monoclonal

opis

recombinant, expressed in HEK 293 cells

linia produktu

ZooMAb® learn more

Postać

lyophilized

masa cząsteczkowa

calculated mol wt 1.35 kDa
observed mol wt ~30 kDa

oczyszczone przez

using Protein A

reaktywność gatunkowa

human, mouse, rat

opakowanie

antibody small pack of 25 μL

charakterystyka ekologicznej alternatywy

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

rozszerzona walidacja

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

metody

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

izotyp

IgG

sekwencja epitopowa

Unknown

numer dostępu Protein ID

numer dostępu UniProt

kategoria ekologicznej alternatywy

Warunki transportu

ambient

temp. przechowywania

2-8°C

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specyficzność

Clone 1K3 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Substance P.

Immunogen

KLH-conjugated linear peptide corresponding to full-length Substance P (11 amino acids).

Zastosowanie

Quality Control Testing

Evaluated by Western Blotting with recombinant Substance P (TAC1) with N-terminal His/ABP tags

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected recombinant Substance P (TAC1) with N-terminal His/ABP tags .

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Substance P in Jurkat cell lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Substance P in human cerebral cortex tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Substance P in SH-SY5Y cells.

Affinity Binding Assay: A representative lot of this antibody bound Substance P with a KD of 5.1 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Substance P, clone 1K3 ZooMAb®, Cat. No. ZRB1506, is a recombinant Rabbit monoclonal antibody that detects Substance P and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Opis wartości docelowych

Substance P, a member of the tachykinin family, is an 11 amino acid peptide that is derived from the Protachykinin-1 (UniProt: P20366) that is encoded by TAC1 gene (Gene ID: 6863) in human. Protachykinin-1 is synthesized with a signal peptide (aa 1-19) and a propeptide (aa 20-56), which are subsequently cleaved off. The resulting peptide can be further cleaved into five different peptides- Substance P (aa 58-68), neuropeptide K (aa 72-107), neurokinin A (aa 98-107), neuropeptide gamma (aa 72-107) and C-terminal-flanking peptide (aa 111-126). Substance P is a potent hypotensive peptide that is most well-studied of the tachykinin family of peptides. Its actions are mediated via binding to NK1 receptors. Substance P is widely distributed in the central and peripheral nervous system of vertebrates. It is also found in association with nerves intrinsic to the gastrointestinal tract arising from the submucous and myenteric plexuses. In the CNS, it participates in various behavioral responses and in regulating neuronal survival and degeneration. It also regulates cardiovascular and respiratory function and is involved in activating the emetic reflex. In the spinal cord, substance P participates in neurotransmission of pain and noxious stimuli and modulates autonomic reflexes. It is also reported to elicit local vasodilatation and alter vascular permeability, which enhances the delivery and accumulation of leukocytes to tissues for the expression of local immune responses. It is also known to stimulate the chemotaxis of lymphocytes, monocytes, neutrophils, and fibroblasts. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: O Connor, TM., et al. (2004). Cell. Physiol. 201(2); 167-180).

Postać fizyczna

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstytucja

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informacje prawne

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.

Nie możesz znaleźć właściwego produktu?  

Wypróbuj nasz Narzędzie selektora produktów.

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej