ZRB1181

Anti-SCARB1 Antibody, clone 3B15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonim(y): CD36, CD36 antigen-like 1, CLA-1, Collagen type I receptor thrombospondin receptor-like 1, LIMPII analogous 1, SR-BI, SRB1, Scavenger receptor class B member 1
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: rabbit
• Poziom jakości: 200
• rekombinowane: expressed in HEK 293 cells
• białko sprzężone: unconjugated
• forma przeciwciała: purified antibody
• klon: recombinant monoclonal
• linia produktu: ZooMAb^® learn more
• Postać: lyophilized
• masa cząsteczkowa: calculated mol wt 60.88 kDa; observed mol wt ~65 kDa
• reaktywność gatunkowa: human
• opakowanie: antibody small pack of 25 μL
• charakterystyka ekologicznej alternatywy: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• rozszerzona walidacja: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• metody: affinity binding assay: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• izotyp: IgG
• numer dostępu UniProt: Q8WTV0
• kategoria ekologicznej alternatywy: , Aligned
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified

Opis

Opis ogólny

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specyficzność

Clone 3B15 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Scavenger receptor class B member 1 (SCARB1). It targets an epitope within 25 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal, extracellular domain of human Scavenger receptor class B member 1 (SCARB1).

Zastosowanie

Quality Control Testing
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SCARB1 in Human adrenal gland tissue lysate.Tested applicationsWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SCARB1 in HeLa Membrane extract.Affinity Binding Assay: A representative lot of this antibody bound SCARB1 peptide with a KD of 5.8 x 10-9 in an affinity binding assay.Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected SCARB1 in human adrenal gland tissue sections.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SCARB1, clone 3B15 ZooMAb, Cat. No. ZRB1181, is a recombinant Rabbit monoclonal antibody that specifically targets SCARB1 and has been tested for use in Affinity Binding Assay, Immunohistochemistry (Paraffin), and Western Blotting.

Opis wartości docelowych

Scavenger receptor class B member 1 (UniProt: Q8WTV0; also known as SCARB1, SRB1, CD36 and LIMPII analogous 1, CLA-1, CD36 antigen-like 1, Collagen type I receptor, thrombospondin receptor-like 1, SR-BI, CD36) is encoded by the SCARB1 (also known as CD36L1, CLA1) gene (Gene ID: 949) in human. SCARB1 is a widely expressed, multi-pass membrane glycoprotein with two cytoplasmic domains (aa 1-11 and 465-552), one extracellular domain (aa 33-443) and two transmembrane domains (aa 12-32 and 444-464). It serves as a receptor for several different ligands such as phospholipids, cholesterol ester, lipoproteins, and phosphatidylserine. It also serves as a receptor for HDL and mediates selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux. It is reported to facilitate the flux of free and esterified cholesterol between the cell surface and ApoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. SCRAB1 is also involved in phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Elevated expression of SCARB1 has been reported in a wide variety of malignant cell lines such as breast, prostate, ovarian, pancreatic, nasopharyngeal, and colorectal cancers. In hepatocytes, it is reported to act as a receptor for hepatitis C virus and facilitates virus entry into cells. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Mooberry, LK., et al. (2016). Front. Pharmacol. 7; 466).

Postać fizyczna

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Rekonstytucja

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Przechowywanie i stabilność

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Informacje prawne

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable