Y2377

2x YT medium

Powder microbial growth medium

• Synonim(y): 2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media
• Kod UNSPSC: 41106225
• NACRES: NA.85

Właściwości

• klasa czystości: for molecular biology
• sterylność: non-sterile
• Postać: powder
• metody: microbiological culture: suitable
• pH: 6.8-7.2(3.1% solution)
• Zastosowanie: food and beverages; microbiology
• temp. przechowywania: room temp
• przydatność: nonselective for Escherichia coli; nonselective for coliforms

Opis

Opis ogólny

2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Zastosowanie

2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

Cechy i korzyści

2xYT powder provides:

• Standard formulation
• An inexpensive alternative to liquid

Komponenty

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl

Uwaga dotycząca przygotowania

1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.

Rekonstytucja

Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)