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Merck

V7259

Sigma-Aldrich

Vascular Endothelial Growth Factor human

≥98% (SDS-PAGE), recombinant, expressed in E. coli, powder, suitable for cell culture

Synonim(y):

VEGF

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About This Item

Numer CAS:
Numer MDL:
Kod UNSPSC:
12352202
NACRES:
NA.77

Nazwa produktu

Vascular Endothelial Growth Factor human, VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture

pochodzenie biologiczne

human

Poziom jakości

rekombinowane

expressed in E. coli

Próba

≥98% (SDS-PAGE)

Formularz

powder

siła działania

1-10 ng/mL ED50/EC50

jakość

endotoxin tested

masa cząsteczkowa

protein 38.2 kDa (as homodimer)

opakowanie

pkg of 10 μg

warunki przechowywania

avoid repeated freeze/thaw cycles

metody

cell culture | mammalian: suitable

zanieczyszczenia

≤0.1 EU/μg

kolor

white

rozpuszczalność

water: soluble

numer dostępu UniProt

temp. przechowywania

−20°C

informacje o genach

human ... VEGFA(7422)

Opis ogólny

Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms.

Zastosowanie

Vascular Endothelial Growth Factor (VEGF) has been used for-
  • the determination of oligodendrocyte precursor cells (OPCs) and luteal cell proliferation/survival
  • inducing proliferation of epithelial cell clusters (ECCs) to be transplanted into mice

Działania biochem./fizjol.

Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen.

VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.

Właściwości fizyczne

Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa

Postać fizyczna

Lyophilized from a sterile filtered buffered solution with no additives.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Produkty

VEGF-A stymuluje wzrost naczyń krwionośnych poprzez proliferację, migrację i przeżycie komórek śródbłonka podczas rozwoju embrionalnego.

Vascular endothelial growth factor (VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF-A (VEGF) is a potent growth factor for blood vessel endothelial cells, showing pleiotropic responses that facilitate cell migration, proliferation, tube formation, and survival. In the developing embryo VEGF-A mRNA is expressed by cells within tissues undergoing capillarization.

Testy angiogenezy oparte na komórkach do analizy tworzenia nowych naczyń krwionośnych do zastosowań w badaniach nad rakiem, regeneracji tkanek i biologii naczyniowej.

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