T7254

N_α-Tosyl-L-lysine chloromethyl ketone hydrochloride

powder, ≥96% (TLC)

• Synonim(y): (3S)-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride, (3S)-7-Amino-1-chloro-3-tosylamino-2-heptanone hydrochloride, TLCK, Tosyl-L-lysyl-chloromethane hydrochloride
• Wzór empiryczny (zapis Hilla): C₁₄H₂₁ClN₂O₃S · HCl
• Numer CAS: 4272-74-6
• Masa cząsteczkowa: 369.31
• Beilstein: 7106867
• Numer WE: 224-266-4
• Numer MDL: MFCD00065395
• Kod UNSPSC: 12352202
• Identyfikator substancji w PubChem: 24278752
• NACRES: NA.77

Właściwości

• product name: N_α-Tosyl-L-lysine chloromethyl ketone hydrochloride, ≥96% (TLC), powder
• pochodzenie biologiczne: synthetic (organic)
• Poziom jakości: 200
• Próba: ≥96% (TLC)
• Postać: powder
• siła działania: 10-100 μM effective concentration
• rozpuszczalność: H₂O: 50 mg/mL
• temp. przechowywania: −20°C
• ciąg SMILES: Cl[H].Cc1ccc(cc1)S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl
• InChI: 1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1
• Klucz InChI: YFCUZWYIPBUQBD-ZOWNYOTGSA-N
• informacje o genach: human ... NFKB1(4790) , NFKB2(4791)

Opis

Zastosowanie

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used:

• in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extract
• as a protease inhibitor in tissue and cell homogenization
• as a trypsin inhibitor to determine the specific protease activity levels

Działania biochem./fizjol.

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli.

Opakowanie

Bottomless glass bottle. Contents are inside inserted fused cone.

Przestroga

Stable at least two years when stored desiccated at −20°C.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves