T6258

Triosephosphate Isomerase from rabbit muscle

Type X, lyophilized powder, ≥3,500 units/mg protein

• Synonim(y): D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI
• Numer CAS: 9023-78-3
• Numer EC enzymu: 5.3.1.1 (BRENDA, IUBMB)
• Numer WE: 232-774-2
• Numer MDL: MFCD00082080
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• typ: Type X
• Postać: lyophilized powder
• aktywność właściwa: ≥3,500 units/mg protein
• masa cząsteczkowa: calculated mol wt 53.2 kDa
• skład: Protein, 60-90% biuret
• obecność zanieczyszczeń: pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
• temp. przechowywania: −20°C

Opis

Opis ogólny

Triosephosphate isomerase (TPI) gene is located on human chromosome 12p13. It is expressed in all tissues. This homodimer of two 27 kDa subunits is highly stable and is made up of 248 amino acids. TPI is catalytically active in its dimeric state.

Zastosowanie

Triosephosphate Isomerase from rabbit muscle has been complexed with p-hydroxymercurybenzoate (PHMB) to denature the protein for hydrophobic interaction chromatography coupled online with chemical vapour atomic fluorescence spectrometry (HIC−CVGAFS).

Triosephosphate isomerase has been used in a study to assess changes in cardiac energy metabolic pathways in overweight rats fed a high-fat diet. Triosephosphate isomerase has also been used in a study to investigate proteomic identification of carbonylated proteins in F344 rat hippocampus after 1-bromopropane exposure.

Działania biochem./fizjol.

Triosephosphate isomerase (TPI) is responsible for the catalysis of the triose phosphate isomers, dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. TPI deficiency is associated with glycolytic enzymopathy. Lack of TPI is also associated with chronic hemolytic anemia, enhanced exposure to bacterial infections, cardiomyopathy and neuromuscular disease.

Opakowanie

Package size based on protein content

Definicja jednostki

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Postać fizyczna

Sulfate-free, contains EDTA and borate buffer salts

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)