T5326

Anti-γ-Tubulin Antibody

mouse monoclonal, GTU-88

• Synonim(y): Przeciwciało przeciw tubulinie
• Numer MDL: MFCD00677366
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• Nazwa produktu: Anti-γ-Tubulin antibody, Mouse monoclonal, clone GTU-88, purified from hybridoma cell culture
• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• forma przeciwciała: purified antibody (purified from hybridoma cell culture)
• rodzaj przeciwciała: primary antibodies
• klon: GTU-88, monoclonal
• Formularz: buffered aqueous solution
• masa cząsteczkowa: antigen 48 kDa
• reaktywność gatunkowa: rat, hamster, chicken, human, bovine, canine, Xenopus, mouse
• opakowanie: antibody small pack of 25 μL
• rozszerzona walidacja: independent; Learn more about Antibody Enhanced Validation
• stężenie: ~1 mg/mL
• metody: immunocytochemistry: 1-2 μg/mL using HeLa cells; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 1-2 μg/mL using total cell extract of chicken fibroblasts
• izotyp: IgG1
• numer dostępu UniProt: P23258
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... TUBG1(7283) ; mouse ... Tubg1(103733) ; rat ... Tubg1(252921)

Opis

Opis ogólny

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulin γ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule.

Specyficzność

The antibody recognizes an epitope located within the N-terminal region of γ-tubulin.

Immunogen

synthetic γ-tubulin peptide, conjugated to KLH

Zastosowanie

Monoclonal Anti-γ-Tubulin antibody produced in mouse has been used in following studies:

• Western blot analysis
• Immunohistochemistry
• Immunocytology

Działania biochem./fizjol.

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)