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Merck

T4174

Sigma-Aldrich

Trypsin-EDTA solution

10 ×, sterile-filtered, BioReagent, suitable for cell culture, 5.0 g porcine trypsin and 2 g EDTA, 4Na per liter of 0.9% sodium chloride

Synonim(y):

Cocoonase, Tryptar, Tryptase

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About This Item

Numer EC enzymu:
Numer MDL:
Kod UNSPSC:
12352204
NACRES:
NA.75

pochodzenie biologiczne

Porcine

Poziom jakości

sterylność

sterile-filtered

linia produktu

BioReagent

Formularz

solution

masa cząsteczkowa

23.4 kDa

stężenie

10 ×

metody

cell culture | mammalian: suitable

zanieczyszczenia

Porcine parvovirus, none detected (9 CFR)

pH

7.0-7.6

Warunki transportu

dry ice

temp. przechowywania

−20°C

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Zastosowanie

The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
Trypsin-EDTA solution is used for the following applications:
  • Used as a supplement in cell culture for their maintenance
  • In harvesting cells grown to confluence
  • to detach lentivirus-transduced macrophages

Działania biochem./fizjol.

Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Komponenty

Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

Przestroga

This product is stored frozen between -10 and -40°C. Repeated cycles of freezing and thawing should be avoided.

Uwaga dotycząca przygotowania

Incubating cells with too high a trypsin concentration for a long period can damage cell membranes and kill the cells. Solubilizing trypsin or diluting it from a concentrated solution should be done with a buffered salt solution contaiing no Ca2+ or Mg2+.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Certyfikaty analizy (CoA)

Lot/Batch Number

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

George R Wendt et al.
eLife, 7 (2018-03-21)
Schistosomes infect more than 200 million people. These parasitic flatworms rely on a syncytial outer coat called the tegument to survive within the vasculature of their host. Although the tegument is pivotal for their survival, little is known about maintenance
Nadja S Katheder et al.
Nature, 541(7637), 417-420 (2017-01-13)
As malignant tumours develop, they interact intimately with their microenvironment and can activate autophagy, a catabolic process which provides nutrients during starvation. How tumours regulate autophagy in vivo and whether autophagy affects tumour growth is controversial. Here we demonstrate, using
Guosong Qiu et al.
Journal of lipid research, 48(2), 385-394 (2006-11-10)
LPL and endothelial lipase (EL) are associated with macrophages in human atherosclerotic lesions, and overexpression of LPL in mouse macrophages is associated with a greater extent of atherosclerosis. To investigate potential mechanisms by which macrophage-derived lipase expression may mediate proatherogenic
Chiao-Chen Chen et al.
Analytical chemistry, 85(7), 3621-3628 (2013-02-21)
Elucidation of epithelial transport across transcellular or paracellular pathways promises to advance the present understanding of ion transport and enables regulation of cell junctions critical to the cell and molecular biology of the epithelium. Here, we demonstrate a new instrumental
Ricardo Rodrigues-Pinto et al.
Scientific reports, 8(1), 12866-12866 (2018-08-29)
The adult nucleus pulposus originates from the embryonic notochord, but loss of notochordal cells with skeletal maturity in humans is thought to contribute to the onset of intervertebral disc degeneration. Thus, defining the phenotype of human embryonic/fetal notochordal cells is

Protokoły

Trypsin is frequently used in cell dissociation from adherent surfaces. We offer a wide variety of trypsin solutions to meet your specific cell line requirements, as well as protocols, troubleshooting ideas, and more.

Trypsyna jest powszechnie stosowana do oddzielania przylegających komórek od powierzchni. Dostępna jest szeroka gama roztworów trypsyny, aby spełnić specyficzne wymagania linii komórkowej.

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