T3195

Anti-γ-Tubulin Antibody

rabbit polyclonal

• Synonim(y): Anty-CCDCBM4, Anty-GCP-1, Anty-TUBG, Anty-TUBGCP1
• Numer MDL: MFCD00677366
• Kod UNSPSC: 12352203
• NACRES: NA.43

Właściwości

• Nazwa produktu: Anti-γ-Tubulin (DQ-19) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
• pochodzenie biologiczne: rabbit
• białko sprzężone: unconjugated
• forma przeciwciała: IgG fraction of antiserum
• rodzaj przeciwciała: primary antibodies
• klon: polyclonal
• Formularz: buffered aqueous solution
• masa cząsteczkowa: antigen 48 kDa
• reaktywność gatunkowa: chicken, human
• metody: indirect immunofluorescence: 1:500 using methanol-acetone fixed, cultured chicken fibroblasts.; western blot: 1:1,000 using whole cell extract of human epidermal carcinoma A431 cell line.
• numer dostępu UniProt: P23258
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... TUBG1(7283) , TUBG2(27175)

Opis

Opis ogólny

Tubulin (48 kDa) is a widely expressed and highly conserved protein within the microtubule organizing centers (MTOCs) or centrosome in eukaryotic cells. It is a member of the tubulin superfamily of proteins which includes α- and β- tubulin and the newly discovered centrosomal-associated proteins, δ- and ε- tubulin. γ-Tubulin is a part of large protein complex containing at least five other proteins and has a shape of a ring (γ-tubulin ring complex, γ-TuRC) that is roughly the same diameter as a microtubule. Its abundance is less than 1% of the level of either α- or β-tubulin. γ-Tubulin shares 28-32% identity with α-tubulin from various organisms, 32-36% identity with β-tubulins and 29-30% identity with δ- and ε tubulins. Some regions including those thought to be involved in GTP) binding are highly conserved among α-, β-, γ-, δ-, and ε-tubulins.

Immunogen

synthetic peptide corresponding to the C-terminus of human γ-tubulin (amino acids 433-451 with N-terminally added lysine), conjugated to KLH.

Zastosowanie

Anti-γ-Tubulin (DQ-19) antibody produced in rabbit has been used in:

• western blotting
• immunocytochemistry
• immunofluorescence

Działania biochem./fizjol.

The proper organization of microtubules is essential for cell division and chromosome segregation, directed cell movement, interphase cytoplasmic organization, and other cytoskeletal functions. γ-Tubulin has an essential role in microtubule nucleation by the centrosomes. It binds to the β-tubulin half of the tubulin molecule, thus establishing the polarity of a microtubule, leaving the α-tubulin half exposed at the plus end. γ-TuRC (γ-tubulin ring complex) binds the microtubule minus ends and is responsible for mediating the link between microtubules and the centrosome.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable