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T2663
Trizma® hydrochloride solution
1 M, BioReagent, for molecular biology
Synonim(y):
Tris hydrochloride solution
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About This Item
Polecane produkty
klasa czystości
for molecular biology
for molecular biology
Poziom jakości
sterylność
0.2 μm filtered
linia produktu
BioReagent
Formularz
solution
stężenie
1 M
zanieczyszczenia
DNase, RNase, NICKase and protease, none detected
pH
7.35-7.45
ciąg SMILES
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
Klucz InChI
QKNYBSVHEMOAJP-UHFFFAOYSA-N
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Opis ogólny
Trizma® hydrochloride solution is a useful biological buffer.
Zastosowanie
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35. It was used to prepare the triton extract. It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis. It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli.
Inne uwagi
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Informacje prawne
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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