SRP0226
NEDD4 Active human
recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)
Synonim(y):
Cell proliferation-inducing gene 53 protein, KIAA0093, PIG53, RPF1, neural precursor cell expressed, developmentally down-regulated 4
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About This Item
Kod UNSPSC:
12352200
NACRES:
NA.32
Polecane produkty
pochodzenie biologiczne
human
rekombinowane
expressed in baculovirus infected insect cells
Próba
≥60% (SDS-PAGE)
Postać
aqueous solution
masa cząsteczkowa
86 kDa
opakowanie
pkg of 20 μg
stężenie
>0.02 mg/mL
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−70°C
informacje o genach
human ... NEDD4(4734)
Opis ogólny
Neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4) has an amino terminal domain, four tryptophan-rich WW domains and a carboxy-terminal homologous to the E6-AP carboxyl terminus (HECT) ubiquitin ligase domain. The gene encoding this protein is localized on human chromosome 15q21.3. Human Nedd4 (GenBank Accession No. NM_006154), amino acids 150-end, with N-terminal DDDDK tag (FLAG) MW = 86kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Zastosowanie
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Działania biochem./fizjol.
Neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4) associates with the PPxY (PY) motifs of the subunits of the human epithelial Na+ channel (hENaC). It is a positive and negative modulator of signaling receptors. The protein is overexpressed in endometrial cancer.
Definicja jednostki
One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.
Postać fizyczna
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
Uwaga dotycząca przygotowania
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
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Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
NEDD4 ubiquitin ligase is a putative oncogene in endometrial cancer that activates IGF-1R/PI3K/Akt signaling.
Zhang Y
Gynecologic Oncology, 139(1), 127-133 (2015)
Oncogenic role of the E3 ubiquitin ligase NEDD4-1, a PTEN negative regulator, in non-small-cell lung carcinomas.
Amodio N
The American Journal of Pathology, 177(5), 2622-2634 (2010)
The Nedd4-1 WW Domain Recognizes the PY Motif Peptide through Coupled Folding and Binding Equilibria.
Panwalkar V
Biochemistry, 55(4), 659-674 (2016)
Yu-Ru Lee et al.
Science (New York, N.Y.), 364(6441) (2019-05-18)
Activation of tumor suppressors for the treatment of human cancer has been a long sought, yet elusive, strategy. PTEN is a critical tumor suppressive phosphatase that is active in its dimer configuration at the plasma membrane. Polyubiquitination by the ubiquitin
Nicholas M Chesarino et al.
PLoS pathogens, 11(8), e1005095-e1005095 (2015-08-12)
Interferon (IFN)-induced transmembrane protein 3 (IFITM3) is a cell-intrinsic factor that limits influenza virus infections. We previously showed that IFITM3 degradation is increased by its ubiquitination, though the ubiquitin ligase responsible for this modification remained elusive. Here, we demonstrate that
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