Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Key Documents

SRP0128

Sigma-Aldrich

DNMT3a Active human

recombinant, expressed in baculovirus infected insect cells

Synonim(y):

DNA (cytosine-5-)-methyltransferase 3A, DNA MTase HsaIIIA, DNMT3A2, M.HsaIIIA

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12352200
NACRES:
NA.32

pochodzenie biologiczne

human

rekombinowane

expressed in baculovirus infected insect cells

Postać

aqueous solution

masa cząsteczkowa

128 kDa

opakowanie

pkg of 10 μg

stężenie

>0.02 mg/mL

numer dostępu UniProt

Warunki transportu

dry ice

temp. przechowywania

−70°C

informacje o genach

human ... DNMT3A(1788)

Opis ogólny

DNMT3A (DNA methyltransferase 3α) is one of three (DNMT1, DNMT3a and DNMT3b) active forms of DNA methyltransferases. This gene is localized to human chromosome 2p23. DNMT3A is one of the three members of the DNMT3A family, of which DNMT3A and DNMT3B are catalytically active and DNMT3L is an inactive paralogue, which has regulatory actions in germ cells.

Zastosowanie

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Działania biochem./fizjol.

DNA methyltransferases are responsible for the addition of a methyl residue to the cytosine group of CpG dinucleotides. DNMT3A (DNA methyltransferase 3α) participates in the gene expression suppression by interacting with histone modifiers, such as polycomb-group (PcG). The interaction between these two proteins is thought to be implicated in cancer pathogenesis. During embryogenesis this enzyme is responsible for the formation of genomic methylation patterns and germ line development. Polymorphisms in this gene might serve as biomarkers to determine the prognosis of gastric cancer patients. Mutations in this gene are linked with Tatton-Brown-Rahman syndrome, an overgrowth syndrome characterized by unique facial appearance, intellectual disability, and increased height.

Postać fizyczna

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Uwaga dotycząca przygotowania

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
This page may contain text that has been machine translated.

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

DNMT3A mutations in acute myeloid leukemia.
Ley TJ et al
The New England Journal of Medicine, 363(25), 2424-2433 (2010)
DNA methyltransferase 3a rs1550117 genetic polymorphism predicts poor survival in gastric cancer patients.
Wang C et al
International Journal of Clinical and Experimental Pathology, 8(11), 14864-14874 (2015)
DNMT3A R882 mutants interact with polycomb proteins to block haematopoietic stem and leukaemic cell differentiation.
Koya J et al
Nature Communications, 7, 10924-10924 (2016)
Tatton-Brown-Rahman syndrome due to 2p23 microdeletion.
Okamoto N et al
American Journal of Medical Genetics. Part A, 170A(5), 1339-1342 (2016)
Structure of Dnmt3a bound to Dnmt3L suggests a model for de novo DNA methylation.
Jia D et al
Nature, 449(7159), 248-251 (2007)

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej