Przejdź do zawartości
Merck
Wszystkie zdjęcia(2)

Key Documents

SRP0108

Sigma-Aldrich

HDAC-6 human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonim(y):

HD6, Histone deacetylase 6, JM21

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(2)

About This Item

Kod UNSPSC:
12352200
NACRES:
NA.32

pochodzenie biologiczne

human

rekombinowane

expressed in baculovirus infected insect cells

Próba

≥80% (SDS-PAGE)

Postać

aqueous solution

masa cząsteczkowa

159 kDa

opakowanie

pkg of 50 μg

numer dostępu NCBI

BC069243

numer dostępu UniProt

Q9UBN7

Warunki transportu

dry ice

temp. przechowywania

−70°C

informacje o genach

human ... HDAC6(10013)

Opis ogólny

HDAC6 (histone deacetylase 6) is a member of the HDAC family of enzyme which are generally linked with gene expression and chromatin dynamics. This protein resides exclusively in the cytoplasm and is present in the perinuclear punctate structures as well as the leading edges of the cell.

Zastosowanie

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Działania biochem./fizjol.

HDAC6 (histone deacetylase 6) functions as a microtubule or tubulin deacetylase, which in turn regulates microtubule-dependent chemotactic cell motility. This protein shows colocalization with p150glued protein, a dynactin component. It contains a ubiquitin binding zinc finger, and interacts with ubiquitinated proteins post-proteasomal inhibition. It is essential for the survival of inflammatory breast cancers (IBCs), though does not function as an oncogene. Treatment of IBC cells with HDAC6 inhibitor ricolinostat (ACY1215) results in the inhibition of IBC cell proliferation both in vitro and in vivo.

Definicja jednostki

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Postać fizyczna

Formulated in 40 mM Tris-HCl pH8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween20, 3mM DTT, and 20% glycerol.

Uwaga dotycząca przygotowania

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
This page may contain text that has been machine translated.

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

The deacetylase HDAC6 regulates aggresome formation and cell viability in response to misfolded protein stress.
Kawaguchi Y et al
Cell, 115(6), 727-738 (2003)
Charlotte Hubbert et al.
Nature, 417(6887), 455-458 (2002-05-25)
Reversible acetylation of alpha-tubulin has been implicated in regulating microtubule stability and function. The distribution of acetylated alpha-tubulin is tightly controlled and stereotypic. Acetylated alpha-tubulin is most abundant in stable microtubules but is absent from dynamic cellular structures such as
HDAC6 activity is a non-oncogene addiction hub for inflammatory breast cancers.
Putcha P
Breast Cancer Research, 17(1), 149-149 (2015)

Produkty

Histone Modification and Chromatin Remodeling | Epigenetics

Epigenetic modifications are thought to occur through two key interconnected processes—DNA methylation and the covalent modification of histones.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej