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Merck

SAE0078

Sigma-Aldrich

MMP-9 pre-activated human

recombinant, ≥1,300 pmol/min/μg, expressed in HEK 293 cells

Synonim(y):

GELB, GELBCLG4B, Gelatinase, Gelatinase B, MANDP2, MMP-9, Matrix Metalloproteinase-10, Type IV collagenase

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About This Item

Numer EC enzymu:
Kod UNSPSC:
12352202
NACRES:
NA.32

pochodzenie biologiczne

human

Poziom jakości

rekombinowane

expressed in HEK 293 cells

Próba

≥95% (SDS-PAGE)

Postać

liquid

masa cząsteczkowa

calculated mol wt 66 kDa
observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation)

stężenie

≥50 μg/mL

numer dostępu UniProt

Zastosowanie

cell analysis

Warunki transportu

dry ice

temp. przechowywania

−20°C

informacje o genach

human ... MMP(4318)

Opis ogólny

50 μg protein, determined by Bradford. The protein concentration is ≥50 μg/ml. for the lot-specific concentration, see Certificate of Analysis.

Działania biochem./fizjol.

MMP-9 is a member of the matrix metalloproteinase (MMP) family of proteins. Proteins of the MMP family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Studies in rhesus monkeys suggest that MMP9 is involved in IL-8 (interleukin-8)-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. Thrombospondins, intervertebral disc proteins, regulate the effective levels of MMP-2 and -9, which are key effectors of extracellular matrix (ECM) remodeling. MMP-9 degrades various substrates including gelatin, collagen types IV and V, and elastin. MMP-9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis, and be regarded as a potential therapeutic target.
As with most MMPs, MMP-9 is secreted as an inactive pro-protein which is activated when cleaved by extracellular proteinases. MMP-9 can be cleaved (and thus activated) in vitro using 4-Aminophenylmercuric acetate (APMA), Cat. No. A9563. MMP-9 is secreted from neutrophils, macrophages, and a number of transformed cells, and is the most complex family member in terms of domain structure and regulation of its activity.
Structurally, MMP9 maybe be divided into five distinct domains: a pro-domain which is cleaved upon activation, a gelatin binding domain consisting of three contiguous fibronectin type II units, a catalytic domain containing the zinc binding site, a proline rich linker region, and a carboxyl terminal hemopexin like domain.
As with most MMPs, MMP-9 is secreted as an inactive pro-protein which is activated when cleaved by extracellular proteinases. This product was cleaved and activated in vitro using 4-Aminophenylmercuric acetate (APMA), Cat. No. A9563.

Inne uwagi

This product was pre-activated in vitro using 4-Aminophenylmercuric acetate (APMA). Thus, it is active and ready for use. In order to save our customers from handling hazardous materials, and for environmental saving, the highly toxic and fatal APMA was removed from the final preparation.

Postać fizyczna

Liquid solution, 0.22 mm filtered, containing 25 mM Tris, 10 mM CaCl2, 0.05% Brij-35
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

Geraldine M Jowett et al.
Nature materials, 20(2), 250-259 (2020-09-09)
Organoids can shed light on the dynamic interplay between complex tissues and rare cell types within a controlled microenvironment. Here, we develop gut organoid cocultures with type-1 innate lymphoid cells (ILC1) to dissect the impact of their accumulation in inflamed

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