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Merck

SAB4200745

Sigma-Aldrich

Anti-Protein A antibody, Mouse Monoclonal

clone SPA-27, purified from hybridoma cell culture

Synonim(y):

Anti-IgG binding protein A, Anti-SpA, Anti-Staphylococcal protein A, Anti-Staphylococcus ayreus protein A

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.46

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

purified from hybridoma cell culture

rodzaj przeciwciała

primary antibodies

klon

SPA-27, monoclonal

Postać

buffered aqueous solution

masa cząsteczkowa

~42 kDa

reaktywność gatunkowa

Staphylococcus aureus

stężenie

~1.0 mg/mL

metody

dot blot: suitable
flow cytometry: suitable
immunoblotting: suitable
immunofluorescence: suitable
immunoprecipitation (IP): 2.5-5 μg/test using purified protein A from Staphylococcus aureus
indirect ELISA: 0.25-0.5 μg/mL using 10 μg/mL of protein A from Staphylococcus aureus for coating. To reduce nonspecific binding we recommend on detection using a secondary anti-mouse, F(ab’)2 fragment antibody

izotyp

IgG1

Warunki transportu

dry ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

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Opis ogólny

Staphylococcus aureus protein A, also known as SpA or IgG binding protein A, is a 42 kDa single chain polypeptide isolated from the cell wall of S. aureus Cowan I strain. It consists of a single polypeptide chain containing five repetitive immunoglobulin-binding domains, rich in aspartic and glutamic acids.Anti-Protein A antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the SPA-27 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c immunized mouse.

Specyficzność

Monoclonal Anti-Protein A specifically recognizes Protein A from Staphylococcus aureus bacteria.

Immunogen

Protein A from Staphylococcus aureus Cowan I strain

Zastosowanie

The antibody may be used in various immunochemical techniques including immunoblotting, dot blot, ELISA, immunofluorescence, immunoprecipitation and flow cytometry (FACS).

Działania biochem./fizjol.

Protein A is considered as a universal reagent in biochemistry and immunology, due to its affinity for the Fc region of many mammalian immunoglobulins. It is used for different applications such as purification of immunoglobulins by affinity chromatography, cell surface studies, radioimmunoassay (RIA), enzyme immunoassay (EIA), immunoprecipitations and many other procedures. It can be used either in its native form, conjugated to various markers or serve as a tag for a specific protein of interest. In addition, protein A has low affinity to mouse monoclonal antibodies from IgG1 isotype. Thus, a monoclonal anti-protein A antibody of the mouse IgG1 isotype has an advantage due to its specific targeting and binding to protein A via its Fab fragment. Monoclonal anti-protein A antibody can serve a useful tool for quantification and visualization of S. aureus bacteria and bacterial contaminations in different examined samples and for detection of protein A tagged proteins.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

This product is for R&D use only, not for drug, household, or other uses. Please consult the material safety data sheet (MSDS) for information regarding hazards and safe handling practices.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Odwiedź Bibliotekę dokumentów

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Molecular Cell, 72(4), 687-699 (2018)
S M Hsu et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29(11), 1349-1353 (1981-11-01)
Conjugated and unlabeled peroxidase antibody methods have proven to be quite satisfactory in localizing sites of antigen-antibody reaction. The use of avidin-biotin-peroxidase complex (ABC), as well as protein A, can contribute significantly to the field of immunohistochemistry. The sensitivity and
Jasdeep S Nanra et al.
Human vaccines & immunotherapeutics, 9(3), 480-487 (2012-12-20)
Staphylococcus aureus can cause severe life threatening invasive diseases. The principal immune effector mechanism by which humans are protected from Gram positive bacteria such as S. aureus is antigen specific antibody- and complement-dependent opsonophagocytosis. This process can be measured in
Rohini Jasavala et al.
Molecular & cellular proteomics : MCP, 6(2), 252-271 (2006-10-21)
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