SAB4200572

Anti-Neuron-Specific Enolase (NSE) antibody, Mouse monoclonal

clone NSE-P2, purified from hybridoma cell culture

• Synonim(y): Monoclonal Anti-2-phospho-D-glycerate hydro-lyase, Monoclonal Anti-2-phospho-D-glycerate hydrolyase, Monoclonal Anti-Enolase 2 (gamma, neuronal), Monoclonal Anti-NSE, Monoclonal Anti-Neural enolase, Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse, Monoclonal Anti-gamma-enolase
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• białko sprzężone: unconjugated
• forma przeciwciała: purified from hybridoma cell culture
• rodzaj przeciwciała: primary antibodies
• klon: NSE-P2, monoclonal
• Postać: buffered aqueous solution
• masa cząsteczkowa: antigen ~47 kDa
• reaktywność gatunkowa: human, rat
• stężenie: ~1 mg/mL
• metody: immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum.; western blot: 2.5-5.0 μg/mL using SH-SY5Y total cell extracts.
• izotyp: IgG1
• numer dostępu UniProt: P09104
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... ENO2(2026)

Opis

Opis ogólny

Monoclonal Anti-Neuron-specific Enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Neuron-specific enolase (NSE) is present in the brain especially in the neuroendocrine cells and neurons. It is a cytosolic enzyme, that exists as a dimer and belongs to the family of enolase enzymes. NSE gene is mapped to human chromosome12p13.31.

Specyficzność

Monoclonal Anti-Neuron-specific Enolase (NSE) recognizes human and rat NSE.

Immunogen

synthetic peptide corresponding to an internal sequence of human NSE.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Zastosowanie

Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse may be used in:

• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry

Działania biochem./fizjol.

Neuron-specific enolase (NSE) catalyzes the production of phosphoenolpyruvate from 2-phospho-D-glycerate in the glycolytic pathway. NSE is a specific neurobiochemical marker of brain damage after traumatic brain injury, hypoxic encephalopathy and stroke. Moreover, NSE has been suggested to be a general circulating marker for neuroendocrine tumors. Interestingly, high levels of NSE were also reported in breast cancer upon exposure to the environmental pollutants arsenite and cadmium.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Przechowywanie i stabilność

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable