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Key Documents

SAB1305538

Sigma-Aldrich

MONOCLONAL ANTI-HIS TAG antibody produced in mouse

clone 6AT18, IgG fraction of antiserum, buffered aqueous solution

Synonim(y):

Mouse Anti-Hexa Histidine tag

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About This Item

Kod UNSPSC:
12352203
NACRES:
NA.43

pochodzenie biologiczne

mouse

Poziom jakości

forma przeciwciała

IgG fraction of antiserum

rodzaj przeciwciała

primary antibodies

klon

6AT18, monoclonal

Postać

buffered aqueous solution

metody

western blot: 1:1000

izotyp

IgG1

numer dostępu UniProt

Warunki transportu

wet ice

temp. przechowywania

−20°C

docelowa modyfikacja potranslacyjna

unmodified

Opis ogólny

Epitope tags consisting of short sequences recognized by well-characterized monoclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal antibody clone 6AT18 provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified from cell lysates by affinity chromatography using Nickel-Sepharose resin. Abgent′s anti-6xHIS monoclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins in cells.

Immunogen

Purified recombinant HIS-tagged fusion protein and poly-HIS peptide were used to produced this monoclonal antibody.

Postać fizyczna

Supplied in PBS with 0.09% (W/V) sodium azide

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Jenifer E Shattuck et al.
Nature communications, 10(1), 3614-3614 (2019-08-11)
Stress granules are membraneless protein- and mRNA-rich organelles that form in response to perturbations in environmental conditions. Stress granule formation is reversible, and persistent stress granules have been implicated in a variety of neurodegenerative disorders, including amyotrophic lateral sclerosis. However
Jialing Bao et al.
Frontiers in cellular and infection microbiology, 11, 694957-694957 (2021-06-08)
Microsporidia are a group of spore-forming, fungus-related pathogens that can infect both invertebrates and vertebrates including humans. The primary infection site is usually digestive tract, but systemic infections occur as well and cause damages to organs such as lung, brain
Emilie Dorard et al.
Molecular neurobiology, 55(7), 5594-5610 (2017-10-07)
Amyloid precursor protein (APP) is cleaved not only to generate the amyloid peptide (Aß), involved in neurodegenerative processes, but can also be metabolized by alpha secretase to produce and release soluble N-terminal APP (sAPPα), which has many properties including the
Pooja Yadav et al.
Retrovirology, 16(1), 12-12 (2019-05-01)
The different interactions between viral proteins and cellular host proteins are required for efficient replication of HIV-1. Various reports implicated host cellular proteins as a key factor that either interact directly with HIV-1 integrase (IN) or get involved in the
Shan Wei et al.
Virus research, 302, 198497-198497 (2021-07-05)
Porcine deltacoronavirus (PDCoV) is an emerging coronavirus that causes vomiting, diarrhea, dehydration, and even death of piglets, resulting in significant losses to the pig industry worldwide. However, the epitopes of PDCoV remain largely unknown. In this study, a monoclonal antibody

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