R354-05A

Rat Aortic Smooth Muscle Cells: RAOSMC, adult

• Kod UNSPSC: 41106514
• NACRES: NA.81

Właściwości

• pochodzenie biologiczne: rat aorta (healthy adult tunica intima and media)
• opakowanie: pkg of 500,000 cells
• producent / nazwa handlowa: Cell Applications, Inc
• tryb wzrostu: Adherent
• morfologia: smooth muscle
• metody: cell culture | mammalian: suitable
• powiązane choroby: cardiovascular diseases
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Opis ogólny

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RAOSMC provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.

RAOSMC from Cell Applications, Inc. have been utilized in dozens of research studies to:

• Provide a gold standard control for smooth muscle markers Ͱ-SMA, calponin and caldesmon expression (Imamura, 2010)
• Elucidate cytokines and growth factors signaling pathways implicated in the molecular regulation of smooth muscle cell proliferation, migration, and overall vascular function (Alexis, 2009; Liu, 2009; Tan, 2009; Ko, 2012; Malabanan, 2012; Santiago, 2012; Newaz, 2013); and demonstrate that peach extract is able to inhibit Ang II-induced intracellular Ca2+ elevation and the generation of ROS, thus showing promise in prevention of cardiovascular diseases (Kono, 2013)
• Investigate extracellular matrix properties and its role in cardiovascular health, repair of damaged vasculature and successful tissue engineering (Chiang, 2009; Kothapalli, 2009a,b,c)
• Demonstrate that hyperglycemia-induced elastin degradation products lead to vascular smooth muscle cell calcification and identify periostin, also implicated in mechanical stress and inflammation responses, as an early marker for this process (Shanmugam, 2013; Sinha, 2013; Yamashita, 2013)
• Develop advanced stent technology, including novel surface materials, and drug delivery systems to prevent restenosis and other occlusive vasculopathies (Taite, 2011; Midwinter, 2012; Li, 2013)

Characterization: positive for smooth muscle cell specific alpha-actin expression.

Pochodzenie linii komórkowej

Aorta

Zastosowanie

Aortic wall contraction, role of smc under normal conditions, blood pressure, distribution of oxygenated blood through systemic circulation.

Komponenty

Rat Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO

Jakość

Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.

Uwaga dotycząca przygotowania

• 2nd passage, >500,000 cells in Rat Smooth Muscle Cell Basal Medium that contains 10% FBS and 10% DMSO
• Can be cultured at least 16 doublings

Rutyna subkultury

Please refer to the RAOSMC Culture Protocol.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable