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R1280
Monoclonal Anti-RPA/p34 antibody produced in mouse
~2 mg/mL, clone 9H8, purified immunoglobulin, buffered aqueous solution
Synonim(y):
Anti-REPA2, Anti-RPA2, Anti-RPA32, Anti-Replication protein A2; 32kDa
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About This Item
białko sprzężone:
unconjugated
application:
ICC
IP
WB
IP
WB
klon:
9H8, monoclonal
reaktywność gatunkowa:
human, monkey
citations:
4
metody:
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.1-0.2 μg/mL using HeLa total cell extract
immunoprecipitation (IP): suitable
western blot: 0.1-0.2 μg/mL using HeLa total cell extract
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
purified immunoglobulin
rodzaj przeciwciała
primary antibodies
klon
9H8, monoclonal
Formularz
buffered aqueous solution
masa cząsteczkowa
antigen ~34 kDa
reaktywność gatunkowa
human, monkey
stężenie
~2 mg/mL
metody
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.1-0.2 μg/mL using HeLa total cell extract
izotyp
IgG1
numer dostępu UniProt
Warunki transportu
dry ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... RPA2(6118)
Powiązane kategorie
Opis ogólny
Monoclonal Anti-RPA/p34 (mouse IgG1 isotype) is derived from the hybridoma 9H8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human RPA/p34. Replication protein A Replication protein A (RPA) complex is a single-stranded DNA-binding protein and it is made up of 70-, 32- and 14-kDa subunits.
Immunogen
purified human RPA/p34.
Zastosowanie
The antibody may be used in:
- immunoblotting (~34 kDa)
- immunocytochemistry
- immunoprecipitation
Działania biochem./fizjol.
Replication protein A (RPA) complex is essential for processes like DNA replication, recombination, and DNA repair pathways. The 32-kDa subunit of human RPA is hyper-phosphorylated in its N-terminus upon cellular DNA damage. This phosphorylation causes a change in RPA conformation that down-regulates its activity in DNA replication but does not affect DNA repair processes. Thus the role of RPA phosphorylation in the cellular response to DNA damage is to help regulate DNA metabolism and promote DNA repair.The RPA complex, once bound to single stranded DNA, recruits the ATR (ataxia-telangiectasia mutated and Rad3-related)-ATRIP (ATR-interacting protein) protein kinase complex that is crucial for the cellular response to replication stress and DNA damage and for ATR-mediated Chk1 activation in human cells. Furthermore, the association of this complex to the single stranded DNA causes the phosphorylation of RAD17 protein that is bound to DNA. Yeast strains deficient in RPA are defective in recruiting Ddc2 (yeast homologue of ATRIP) and thus down stream proteins are not phosphorylated. Replication protein A binds with the ssDNA with great affinity and interacts specifically with multiple proteins.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Sensing DNA damage through ATRIP recognition of RPA-ssDNA complexes
Zou L and Elledge S J
Science (New York, N.Y.), 300(5625), 1542-1548 (2003)
W Q Qiu et al.
The Journal of biological chemistry, 271(14), 8443-8451 (1996-04-05)
Progressive cerebral deposition of the amyloid beta-peptide (Abeta) is an early and constant feature of Alzheimer's disease. Abeta is derived by proteolysis from the beta-amyloid precursor protein. beta-Amyloid precursor protein processing and the generation of Abeta have been extensively characterized
Replication protein A phosphorylation and the cellular response to DNA damage
Binz S K, et al.
DNA Repair, 3(8-9), 1015-1024 (2004)
E Stigger et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(2), 579-583 (1994-01-18)
Human single-stranded DNA-binding protein (HSSB), also known as replication protein A, is composed of a 70-kDa single-stranded DNA-binding subunit (p70) and 34-kDa and 11-kDa (p34 and p11, respectively) subunits of unknown functions. We have examined interactions among the HSSB subunits
Beáta E Jády et al.
Molecular biology of the cell, 17(2), 944-954 (2005-12-02)
Telomerase is a ribonucleoprotein enzyme that counteracts replicative telomere erosion by adding telomeric sequence repeats onto chromosome ends. Despite its well-established role in telomere synthesis, telomerase has not yet been detected at telomeres. The RNA component of human telomerase (hTR)
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