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P7899
Anti-Peroxidase antibody produced in rabbit
fractionated antiserum, lyophilized powder
Synonim(y):
Rabbit Anti HRP
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About This Item
Polecane produkty
pochodzenie biologiczne
rabbit
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
fractionated antiserum
rodzaj przeciwciała
primary antibodies
klon
polyclonal
Formularz
lyophilized powder
reaktywność gatunkowa
horseradish
metody
indirect ELISA: 1:100,000-1:200,000
temp. przechowywania
2-8°C
docelowa modyfikacja potranslacyjna
unmodified
Opis ogólny
Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.
Peroxidase is a hemoprotein.
The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.
Immunogen
Purified peroxidase from horseradish
Zastosowanie
Anti-Peroxidase antibody produced in rabbit has been used in:
- immunoblot assays
- enzyme-linked-immunosorbent assay (ELISA)
- immunoprecipitation
Działania biochem./fizjol.
Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.
Postać fizyczna
Lyophilized from 0.01 M phosphate buffered saline, pH 7.2
Rekonstytucja
Reconstitute with deionized water.
Komentarz do analizy
Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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