P7871
Anti-phospho-PAK1/2/3 (pSer141) antibody produced in rabbit
affinity isolated antibody, buffered aqueous glycerol solution
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About This Item
pochodzenie biologiczne
rabbit
Poziom jakości
białko sprzężone
unconjugated
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
primary antibodies
klon
polyclonal
Postać
buffered aqueous glycerol solution
reaktywność gatunkowa
human
metody
western blot: 1:1,000 using Cos cells transiently transfected with PAK1, PAK2, or PAK3, and co-transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation
numer dostępu UniProt
Warunki transportu
wet ice
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
phosphorylation (pSer141)
informacje o genach
human ... PAK1(5058)
Opis ogólny
p21-activated kinases are a family of highly conserved serine/threonine protein kinases that are activated by small GTPases of Cdc42 and Rac families. There are 2 groups of PAKs in mammals, group A and B. The group A members are three isoforms, PAK1, PAK2, and PAK3 (also known as α-PAK, γ-PAK, and β-PAK, respectively). PAKs are actively involved in regulation of MAPK pathways by phosphorylation of Raf-1 and MEK-1. They also regulate cytoskeletal reorganization by interaction with GTPases. Autophosphorylation of serine 141 (serine 144 for PAK1 and serine 139 for PAK3), catalyzed by Cdc42, is required for activation of PAK.
The antibody detects human PAK1 (46% homologous), PAK2, and PAK3 (46% homologous). Other species have not been tested.
The antibody detects human PAK1 (46% homologous), PAK2, and PAK3 (46% homologous). Other species have not been tested.
Specyficzność
The antibody is highly selective for the phosphorylated form of PAK and can be used to detect an active form of PAK1, as well as PAK2 and PAK3.
Immunogen
synthetic phosphopeptide derived from the region of human PAK2 that contains serine 141.
Zastosowanie
A working dilution of 1:1000 is recommended for immunoblotting detection in Cos cells transiently transfected with PAK1, PAK2, or PAK3, and co-transfected with V12 Cdc42 and grown either as adherent cultures to activate PAK, or placed in suspension to suppress PAK activation.
Postać fizyczna
Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3, with 50% glycerol, 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Clemens Hofmann et al.
Journal of cell science, 117(Pt 19), 4343-4354 (2004-08-28)
p21-activated kinases (Paks) are a highly conserved family of enzymes that bind to and are activated by small GTPases of the Cdc42 and Rac families. With the notable exception of plants, nearly all eukaryotes encode one or more Pak genes
Gholam H Pezeshkpour et al.
Genes & cancer, 4(7-8), 315-324 (2013-10-30)
CRK (c-Crk) as an adaptor protein is involved in several oncogenic signal transduction pathways, conveying oncogenic signals to its downstream effectors and thereby affecting multiple cellular processes including proliferation, differentiation, and migration. For example, we have observed that CRK expression
Zhou-shen Zhao et al.
The Biochemical journal, 386(Pt 2), 201-214 (2004-11-19)
The Rho GTPases are a family of molecular switches that are critical regulators of signal transduction pathways in eukaryotic cells. They are known principally for their role in regulating the cytoskeleton, and do so by recruiting a variety of downstream
Christy C Ong et al.
Oncotarget, 2(6), 491-496 (2011-06-10)
The p21-activated kinases (PAKs) are central players in growth factor signaling networks and morphogenetic processes that control proliferation, cell polarity, invasion and actin cytoskeleton organization. This raises the possibility that interfering with PAK activity may produce significant anti-tumor activity. In
Mengwei Zang et al.
The Journal of biological chemistry, 277(6), 4395-4405 (2001-12-26)
Activation of Raf-1 is a complex process in which phosphorylation of Ser(338)-Tyr(341) is a critical step. Previous studies have shown that Pak1/2 is implicated in both Ras-dependent and -independent activation of Raf-1 by phosphorylating Raf Ser(338). The present study explores
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