P5994

Phosphatase substrate

40 mg tablets

• Synonim(y): 4-Nitrophenyl phosphate disodium salt hexahydrate, pNPP disodium salt hexahydrate
• Wzór liniowy: O₂NC₆H₄OP(O)(ONa)₂ · 6H₂O
• Numer CAS: 333338-18-4
• Masa cząsteczkowa: 371.14
• Numer WE: 224-246-5
• Numer MDL: MFCD00066288
• Kod UNSPSC: 12352204
• Identyfikator substancji w PubChem: 24898723
• NACRES: NA.54

Właściwości

• Postać: tablet
• stężenie: 36-44 mg/tablet
• zanieczyszczenia: p-nitrophenol, essentially free
• rozpuszczalność: H₂O: soluble, clear, colorless to yellow (1 tablet in 8 mL of water.)
• temp. przechowywania: −20°C
• ciąg SMILES: O.O.O.O.O.O.[Na+].[Na+].[O-][N+](=O)c1ccc(OP([O-])([O-])=O)cc1
• InChI: 1S/C6H6NO6P.2Na.6H2O/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;;;;;;;/h1-4H,(H2,10,11,12);;;6*1H2/q;2*+1;;;;;;/p-2
• Klucz InChI: KAKKHKRHCKCAGH-UHFFFAOYSA-L

Opis

Opis ogólny

Human phosphatase-substrate networks are mapped based on the literature and protein-protein interaction (PPI) database. Based on gene expression data, the possible network of phosphatase-substrate-kinase is predicted.

Zastosowanie

Phosphatase is a chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. It is been used for the colorimetric detection of CTB fusion proteins. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Phosphatase substrate has been used as a substrate in alkaline phosphatase assay in serum samples, bone marrow stromal cells and human mesenchymal stem cells (hMSC).

The substrate has been used to determine the alkaline phosphatase (ALP) activity in human osteoblast-like Saos-2 cell lysates. ALP acts as an early marker of osteoblast differentiation that is expressed just before matrix mineralization begins.

Chromogenic substrate for use with alkaline and acid phosphatase ELISA procedures. Produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 N NaOH and read at 405 nm.

Ilość

Contains 40 mg substrate per tablet

Uwaga dotycząca przygotowania

Dissolve tablets to the desired concentration in either of the following buffers: 0.1 M glycine, pH 10.4, with 1 mM MgCl₂ and 1 mM ZnCl₂ or 1 M diethanolamine, pH 9.8, with 0.5 mM MgCl₂. Typically, a pNPP concentration of 1 mg/mL is used.

Inne uwagi

Substrate for the determination of acid and alkaline phosphatases.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)