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P5459
Protease from Bacillus licheniformis
glycerol solution (50%)
Synonim(y):
Proteinaza z Bacillus licheniformis, Subtylizyna A
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About This Item
Numer CAS:
Numer MDL:
Kod UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Polecane produkty
pochodzenie biologiczne
Bacillus licheniformis
Poziom jakości
Formularz
glycerol solution (50%)
aktywność właściwa
≥7 units/mg protein (biuret)
stężenie
≥50 mg/mL
rozpuszczalność
0.85% sodium chloride: soluble, clear (Diluted)
H2O: miscible
temp. przechowywania
2-8°C
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Opis ogólny
The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.
Zastosowanie
Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle.
Działania biochem./fizjol.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.
Opakowanie
Package size based on protein content.
Definicja jednostki
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 2
Środki ochrony indywidualnej
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Protokoły
To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.
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