Wszystkie zdjęcia(2)
Key Documents
P1860
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution
Synonim(y):
Protease Inhibitor Solution, protease inhibitor
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Polecane produkty
product name
Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
Poziom jakości
Postać
DMSO solution
Warunki transportu
dry ice
temp. przechowywania
−20°C
Szukasz podobnych produktów? Odwiedź Przewodnik dotyczący porównywania produktów
Opis ogólny
Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.
Specyficzność
Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
Zastosowanie
Protease Inhibitor Cocktail has been used:
- as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
- to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
- in trypsin solution for trypsin-inhibited studies
- as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot
This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.
Działania biochem./fizjol.
Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.
Cechy i korzyści
- Broad specificity
- Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
- Contains no metal chelators: Ensures compatibility with downstream applications
- Convenient packaging: 1 mL in glass bottle
- Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.
Komponenty
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
Bestatin
E-64
Leupeptin
Pepstatin A
Przestroga
After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
Ilość
Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.
Postać fizyczna
Solution in DMSO (D 2650, Hybri-Max).
Inne uwagi
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
This page may contain text that has been machine translated.
produkt powiązany
Numer produktu
Opis
Cennik
Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
188.6 °F
Temperatura zapłonu (°C)
87 °C
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Klienci oglądali również te produkty
Raymond L Warters et al.
Radiation research, 172(1), 82-95 (2009-07-08)
Although skin is usually exposed during human exposures to ionizing radiation, there have been no thorough examinations of the transcriptional response of skin fibroblasts and keratinocytes to radiation. The transcriptional response of quiescent primary fibroblasts and keratinocytes exposed to from
Takuma Shiratori et al.
Journal of immunology (Baltimore, Md. : 1950), 200(1), 218-228 (2017-11-17)
As osteoclasts have the central roles in normal bone remodeling, it is ideal to regulate only the osteoclasts performing pathological bone destruction without affecting normal osteoclasts. Based on a hypothesis that pathological osteoclasts form under the pathological microenvironment of the
Matthew Townsend et al.
The Journal of physiology, 572(Pt 2), 477-492 (2006-02-14)
The accumulation of amyloid beta-protein (Abeta) in brain regions serving memory and cognition is a central pathogenic feature of Alzheimer's disease (AD). We have shown that small soluble oligomers of human Abeta that are naturally secreted by cultured cells inhibit
Vanessa Neuhaus et al.
PloS one, 8(8), e71728-e71728 (2013-08-24)
Annual outbreaks of influenza infections, caused by new influenza virus subtypes and high incidences of zoonosis, make seasonal influenza one of the most unpredictable and serious health threats worldwide. Currently available vaccines, though the main prevention strategy, can neither efficiently
Rachel Kaminsky et al.
Developmental cell, 17(5), 724-735 (2009-11-20)
Sumoylation is a reversible posttranslational modification that plays roles in many processes, including transcriptional regulation, cell division, chromosome integrity, and DNA damage response. Using a proteomics approach, we identified approximately 250 candidate targets of sumoylation in C. elegans. One such
Produkty
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
Powiązane treści
Wybierz różne typy inhibitorów proteaz w zależności od potrzeb, aby zapobiec degradacji białek podczas izolacji i charakteryzacji oraz zabezpieczyć białka podczas przygotowywania próbek.
Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.
Skontaktuj się z zespołem ds. pomocy technicznej