P0270

Calmodulin from bovine heart

lyophilized powder, 2,500-10,000 units/mg protein

• Synonim(y): Phosphodiesterase 3′:5′-cyclic nucleotide activator from bovine heart
• Numer CAS: 77107-46-1
• Numer MDL: MFCD00131869
• Kod UNSPSC: 12352202
• NACRES: NA.61

Właściwości

• pochodzenie biologiczne: bovine heart
• Poziom jakości: 200
• Postać: lyophilized powder
• aktywność właściwa: 2,500-10,000 units/mg protein
• skład: Protein, ≥85% Lowry
• metody: calcium flux assay: suitable
• numer dostępu UniProt: P62157
• temp. przechowywania: −20°C
• informacje o genach: cow ... CALM3(520277)

Opis

Zastosowanie

This is the protein activator required for the Ca²⁺ dependent activation of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520.

Działania biochem./fizjol.

Ca²⁺ binding protein that is required for activation of cyclic nucleotide-dependent phosphodiesterase. It is also a cofactor/activator of nitric oxide synthase, calcineurin, and many kinases including ATPase, myosin light chain kinase, and CAM kinase I, II, and III. It mediates ryanodine receptor activation by cyclic ADP ribose and is involved in intracellular Ca²⁺ homeostasis.

Definicja jednostki

One unit will stimulate 0.008 activated unit of phosphodiesterase, 3′:5′-cyclic nucleotide, P0520, in a 3 mL reaction volume at pH 7.5 and 30 °C, to 50% of the maximum activity of the enzyme when saturated with activator, in the presence of 0.01 mM Ca²⁺.

Postać fizyczna

Lyophilized powder containing buffer salts as imidazole and magnesium sulfate

Uwaga dotycząca przygotowania

Purified by a modification of the procedure of Teo, T.S., et al., J. Biol. Chem., 248, 588 (1973).

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)