OGS3213
PSF-CMV-PURO-NH2-FLAG® - N-TERMINAL FLAG® TAG MAMMALIAN PLASMID
plasmid vector for molecular cloning
Synonim(y):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
About This Item
Kod UNSPSC:
12352200
NACRES:
NA.85
Polecane produkty
znacznik
FLAG® tagged
Postać
buffered aqueous solution
masa cząsteczkowa
size 6173 bp
selekcja bakterii
kanamycin
selekcja komórek ssaków
puromycin
Pochodzenie replikacji
pUC (500 copies)
Rozszczepienie peptydów
no cleavage
Lokalizacja znacznika peptydowego
N-terminal
Promotor
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
gen reporterowy
none
Warunki transportu
ambient
temp. przechowywania
−20°C
Opis ogólny
This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.
About the Peptide Tag:This plasmid contains an n-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
About the Peptide Tag:This plasmid contains an n-terminal FLAG epitope tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: DYKDDDDK.
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.
Sekwencja
To view sequence information for this product, please visit the product page
Komentarz do analizy
To view the Certificate of Analysis for this product, please visit www.oxgene.com
Informacje prawne
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Cennik
Kod klasy składowania
12 - Non Combustible Liquids
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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