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Merck

N1127

Sigma-Aldrich

2-Nitrophenyl β-D-galactopyranoside

≥98% (enzymatic)

Synonim(y):

ONPG , o-Nitrophenyl β-D-galactopyranoside, ONPG

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About This Item

Wzór empiryczny (zapis Hilla):
C12H15NO8
Numer CAS:
Masa cząsteczkowa:
301.25
Beilstein:
92207
Numer WE:
Numer MDL:
Kod UNSPSC:
12352200
Identyfikator substancji w PubChem:
NACRES:
NA.52

klasa czystości

for molecular biology

Poziom jakości

Próba

≥98% (enzymatic)

Postać

powder

temp. przechowywania

−20°C

ciąg SMILES

OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1

Klucz InChI

KUWPCJHYPSUOFW-YBXAARCKSA-N

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Opis ogólny

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Zastosowanie

2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.

Działania biochem./fizjol.

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Zasada

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Rekonstytucja

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.

Substraty

Chromogenic substrate for β-galactosidase
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Receptor-mediated transport of DNA into eukaryotic cells.
M Cotten et al.
Methods in enzymology, 217, 618-644 (1993-01-01)
Superimposition of temperature regulation on yeast promoters.
A Z Sledziewski et al.
Methods in enzymology, 185, 351-366 (1990-01-01)
Steven T Bruckbauer et al.
PloS one, 14(1), e0199482-e0199482 (2019-01-24)
We have previously generated four replicate populations of ionizing radiation (IR)-resistant Escherichia coli though directed evolution. Sequencing of isolates from these populations revealed that mutations affecting DNA repair (through DNA double-strand break repair and replication restart), ROS amelioration, and cell
D R Henderson et al.
Clinical chemistry, 32(9), 1637-1641 (1986-09-01)
Genetic engineering of beta-galactosidase (EC 3.2.1.23) has led to the development of a new homogeneous assay system, CEDIA. The Z gene of the lac operon of Escherichia coli encodes a large enzymatically inactive polypeptide that spontaneously aggregates and folds to
Amy H Camp et al.
Genes & development, 23(8), 1014-1024 (2009-04-25)
Spore formation by Bacillus subtilis takes place in a sporangium consisting of two chambers, the forespore and the mother cell, which are linked by pathways of intercellular communication. One pathway, which couples the activation of the forespore transcription factor sigma(G)

Produkty

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in a heritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Griffith in 1928. Transforming principle of DNA was demonstrated by Avery et al. in 1944.

Protokoły

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

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