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MAK175

Sigma-Aldrich

Cholesterol Extraction Kit

greener alternative

sufficient for 40 extractions

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About This Item

Kod UNSPSC:
12161503
NACRES:
NA.25

zastosowanie

sufficient for 40 extractions

charakterystyka ekologicznej alternatywy

Waste Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Zastosowanie

cosmetics
food and beverages
sample preservation

kategoria ekologicznej alternatywy

Aligned,

temp. przechowywania

room temp

Powiązane kategorie

Opis ogólny

Cholesterol is a structural component of cell membranes and a precursor to hormones. The Cholesterol Extraction Kit enables the extraction of sterols including cholesterol from biological samples in two simple steps. The extracted sterols can then be saponified, derivatized, and quantified using gas chromatography (GC) with flame-ionization detection (FID) or gas chromatography/mass spectrometry (GC/MS).

Przydatność

This kit is suitable for the extraction of cholesterol and cholesterol-like compounds (sterols) from whole blood, serum, biological tissue and food samples.

Zasada

Sterols including cholesterol are typically extracted using a dual solvent partition system containing a lipophilic solvent and an aqueous solvent. Sterols are retained in the lower chloroform phase, whereas, aqueous-soluble compounds are retained in the methanol-water layer. The sample is then centrifuged and the bottom chloroform layer is transferred with a pipette to another test tube. An aliquot of the transferred layer is then saponified with methanolic NaOH or KOH, and then derivatized with trimethylsilyl chloride (chlorotrimethylsilane). The formed cholesterol-TMS ester is reconstituted with hexane and injected directly into a GC-FID or GC/MS system for quantification.

The Cholesterol Extraction Kit shortens the extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent containing the internal standard, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing sterols. A portion of the total sterol extract can be saponified and derivatized for GC-FID or GC/MS analysis as described in the procedure.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods.Find details here.

Komentarz do analizy

Click on the following link for a demo: Easy to Use High-Throughput Kits for Cholesterol and Lipid Extractions

Inne uwagi

Easy to Use High-Throughput Kits for Cholesterol and Lipid Extractions. Watch the Video to learn more and to see how the technology works.
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Hasło ostrzegawcze

Danger

Klasyfikacja zagrożeń

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 1 - STOT SE 3

Organy docelowe

Central nervous system, Eyes, Liver,Kidney

Kod klasy składowania

3 - Flammable liquids

Temperatura zapłonu (°F)

49.5 °F - closed cup

Temperatura zapłonu (°C)

9.7 °C - closed cup

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
SLBM3376V
SLBL3902V
SLBN5323V
MKBW2217V

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Yahong Zhao et al.
The Journal of biological chemistry, 298(3), 101718-101718 (2022-02-14)
Peripheral myelination is a complicated process, wherein Schwann cells (SCs) promote the formation of the myelin sheath around the axons of peripheral neurons. Fibroblasts are the second resident cells in the peripheral nerves; however, the precise function of fibroblasts in
Cene Skubic et al.
Molecules (Basel, Switzerland), 25(18) (2020-09-13)
We developed a simple and robust liquid chromatographic/mass spectrometric method (LC-MS) for the quantitative analysis of 10 sterols from the late part of cholesterol synthesis (zymosterol, dehydrolathosterol, 7-dehydrodesmosterol, desmosterol, zymostenol, lathosterol, FFMAS, TMAS, lanosterol, and dihydrolanosterol) from cultured human hepatocytes
Laura Inés Elvira-Torales et al.
International journal of molecular sciences, 20(7) (2019-04-17)
The purpose of this work was to evaluate the effect of dietary carotenoids from spinach on the inflammation and oxidative stress biomarkers, liver lipid profile, and liver transcriptomic and metabolomics profiles in Sprague-Dawley rats with steatosis induced by a high-fat
Laura Inés Elvira-Torales et al.
Antioxidants (Basel, Switzerland), 9(11) (2020-10-30)
Non-alcoholic fatty liver disease (NAFLD) is currently a serious and growing clinical problem in developed and developing countries and is considered one of the most frequent chronic liver diseases in the world. The aim of this study was to evaluate

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