Przejdź do zawartości
Merck
Wszystkie zdjęcia(1)

Dokumenty

LYSISO1

Sigma-Aldrich

Lysosome Isolation Kit

sufficient for 25 g (tissue), sufficient for 20 mL (packed cells), enrichment of lysosomes from tissues and packed cells

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych
Wszystkie zdjęcia(1)

About This Item

Numer WE:
233-140-8
Kod UNSPSC:
12352200
NACRES:
NA.32

Poziom jakości

200

zastosowanie

sufficient for 20 mL (packed cells)
 sufficient for 25 g (tissue)

metody

centrifugation: suitable
fractionation: suitable

temp. przechowywania

2-8°C

Opis ogólny

Lysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells. They contain many acid hydrolases that take part in protein degradation in the cell. Lysosomes also contain lipases, nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs, Gaucher, Hunter disease and others. They also contribute to maintaining cellular homeostasis. Malfunctions in this organelle directly impact cell behavior and fate. Lysosomes may also be involved in other cellular processes such as Albinism and aging.

Zastosowanie

Lysosome Isolation Kit has been used:
  • in the isolation of cytosol and lysosome
  • to obtain enriched light and heavy lysosomal fractions from cultured cells
  • in the isolation of lysosomes of mouse bone marrow macrophages (BMMs)
  • in lysosome purification

The Lysosome Isolation Kit provides a method for isolating lysosomes from animal tissues and from cultured cells by differential centrifugation followed by density gradient centrifugation and/or calcium precipitation. The presence of lysosomes can be measured by assaying the activity of the lysosomal marker Acid Phosphatase, with the Acid Phosphatase Assay Kit (Cat. No. CS0740). Separation from other organelles can be measured using the appropriate marker detection kits available from Sigma.

Cechy i korzyści

  • Generates functional organelles for metabolism and disease research
  • Includes protease inhibitor to manage degradation
  • Measures integrity with included Neutral Red dye
  • Choose from multiple options to obtain enriched fractions
  • Enriched fractions for intact and functional lysosomes from tissues and cells
  • Suitable for functional studies including protein degradation
This page may contain text that has been machine translated.

Elementy zestawu są też dostępne oddzielnie

Numer produktu
Opis
Karta charakterystyki
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLKarta charakterystyki

Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H315,H319

Klasyfikacja zagrożeń

Eye Irrit. 2 - Skin Irrit. 2

Kod klasy składowania

10 - Combustible liquids

Temperatura zapłonu (°F)

188.6 °F

Temperatura zapłonu (°C)

87 °C

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Facilitated mitochondrial import of anti-viral and anti-cancer nucleoside drugs by human equilibrative nucleoside transporter-3 (hENT3)
Govindarajan R, et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology (2009)
Activation of Nlrp3 inflammasomes enhances macrophage lipid-deposition and migration: implication of a novel role of inflammasome in atherogenesis
Li X, et al.
PLoS ONE, 9(1), e87552-e87552 (2014)
Glycine N-methyltransferase deficiency affects Niemann-Pick type C2 protein stability and regulates hepatic cholesterol homeostasis
Liao YJ, et al.
Molecular Medicine, 18(3), 412-422 (2012)
Xiaowei Ma et al.
ACS nano, 5(11), 8629-8639 (2011-10-07)
Development of nanotechnology calls for a comprehensive understanding of the impact of nanomaterials on biological systems. Autophagy is a lysosome-based degradative pathway which plays an essential role in maintaining cellular homeostasis. Previous studies have shown that nanoparticles from various sources
Quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections
Xu B, et al.
Molecular Immunology, 87(1), 122-131 (2017)
Wyświetl wszystkie powiązane dokumenty

Produkty

Izolacja organelli

Wirowanie oddziela organelle na podstawie rozmiaru, kształtu i gęstości, ułatwiając frakcjonowanie subkomórkowe w różnych próbkach.

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej