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L9267

Sigma-Aldrich

Lectin from Lens culinaris (lentil)

lyophilized powder

Synonim(y):

Lens culinaris agglutinin, LcH

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About This Item

Kod UNSPSC:
12352202
NACRES:
NA.32

Postać

lyophilized powder

Poziom jakości

siła działania

<16 μg per mL agglutination activity

zanieczyszczenia

salt, free

temp. przechowywania

2-8°C

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Opis ogólny

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

Zastosowanie

Lectin from Lens culinaris (lentil) is used for size- or charge-based separation of proteins with the ANA. (Anisotropic Nanofilter Array: ANA).

Działania biochem./fizjol.

LcH is not blood group specific. It has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. LcH is comprised of two isomers, LcH-A (mol. wt. 49,000) and LcH-B (mol. wt. 49,000). LcH-A is mitogenic. Conjugates are prepared from purified lectin.

Jakość

Contains two major bands on electrophoresis corresponding to isolectins LcH-A and LcH-B.

Uwaga dotycząca przygotowania

Highly purified by affinity chromatography.

Komentarz do analizy

Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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