L9267
Lectin from Lens culinaris (lentil)
lyophilized powder
Synonim(y):
Lens culinaris agglutinin, LcH
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About This Item
Kod UNSPSC:
12352202
NACRES:
NA.32
Polecane produkty
Postać
lyophilized powder
siła działania
<16 μg per mL agglutination activity
zanieczyszczenia
salt, free
temp. przechowywania
2-8°C
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Opis ogólny
Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.
Zastosowanie
Lectin from Lens culinaris (lentil) is used for size- or charge-based separation of proteins with the ANA. (Anisotropic Nanofilter Array: ANA).
Działania biochem./fizjol.
LcH is not blood group specific. It has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. LcH is comprised of two isomers, LcH-A (mol. wt. 49,000) and LcH-B (mol. wt. 49,000). LcH-A is mitogenic. Conjugates are prepared from purified lectin.
Jakość
Contains two major bands on electrophoresis corresponding to isolectins LcH-A and LcH-B.
Uwaga dotycząca przygotowania
Highly purified by affinity chromatography.
Komentarz do analizy
Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, type N95 (US)
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