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L8777
Lectin from Phytolacca americana (pokeweed)
lyophilized powder, BioReagent, suitable for cell culture
Synonim(y):
PWM, Pokeweed mitogen
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About This Item
Polecane produkty
sterylność
aseptically filled
Poziom jakości
linia produktu
BioReagent
Postać
lyophilized powder
siła działania
≤0.3 μg/mL mitogenic activity
metody
cell culture | mammalian: suitable
rozpuszczalność
PBS: 1 mg/mL
temp. przechowywania
2-8°C
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Opis ogólny
Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.
Zastosowanie
Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).
Działania biochem./fizjol.
Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.
Jakość
Partially purified TCA precipitate.
Essentially salt-free.
Essentially salt-free.
Komentarz do analizy
Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Resp. Sens. 1 - Skin Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certyfikaty analizy (CoA)
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