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L1412

Sigma-Aldrich

Lysing Enzymes from Trichoderma harzianum

lyophilized powder

Synonim(y):

Glucanex®

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About This Item

Kod UNSPSC:
12352204

Postać

lyophilized powder

aktywność właściwa

≥10 units/g

Zastosowanie

diagnostic assay manufacturing

temp. przechowywania

2-8°C

Zastosowanie

Lysing Enzymes from Trichoderma harzianum is suitable retrieve DNA plugs from agarose gels. It has been used:
  • for protoplast isolation
  • for hydrolyzing poly(1-3)-glucose of the yeast cell wall glucan prior to yeast spheroplast transformation
  • for the lysis of Aspergillus niger vegetative cells

Działania biochem./fizjol.

Lysing Enzymes from Trichoderma harzianum is called Glucanex. It comprised of mixture of isoenzymes with β-1, 3 glucanase activity. Lysing Enzymes from Trichoderma harzianum hydrolyzes yeast cell wall oligosaccharides to glucan for protoplast preparation. Its stability is influenced by pH changes.

Jakość

Contains β-glucanase, cellulase, protease, and chitinase activities

Definicja jednostki

One unit will hydrolyze casein to produce color equivalent to 1.0 micromole (181 μg) of tyrosine per minute at ph 7.5 at 37 deg C (color byfolin-ciocalteu reagent).

Informacje prawne

A product of Novozyme Corp.
Glucanex is a registered trademark of Novozymes Corp.
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Kod klasy składowania

13 - Non Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Katia Cristina Dantas et al.
PloS one, 13(1), e0190408-e0190408 (2018-01-18)
Although early and rapid detection of histoplasmosis is essential to prevent morbidity and mortality, few diagnostic tools are available in resource-limited areas, especially where it is endemic and HIV/AIDS is also epidemic. Thus, we compared conventional and molecular methods to
Thomas Leisen et al.
PLoS pathogens, 16(8), e1008326-e1008326 (2020-08-18)
CRISPR/Cas has become the state-of-the-art technology for genetic manipulation in diverse organisms, enabling targeted genetic changes to be performed with unprecedented efficiency. Here we report on the first establishment of robust CRISPR/Cas editing in the important necrotrophic plant pathogen Botrytis
Elena Wiederhold et al.
Molecular & cellular proteomics : MCP, 8(2), 380-392 (2008-11-13)
Transport of solutes between the cytosol and the vacuolar lumen is of crucial importance for various functions of vacuoles, including ion homeostasis; detoxification; storage of different molecules such as amino acids, phosphate, and calcium ions; and proteolysis. To identify proteins
Xiao-Lian Yuan et al.
Molecular genetics and genomics : MGG, 279(1), 11-26 (2007-10-06)
The expression of inulinolytic genes in Aspergillus niger is co-regulated and induced by inulin and sucrose. We have identified a positive acting transcription factor InuR, which is required for the induced expression of inulinolytic genes. InuR is a member of
Qiang Zhang et al.
Molecular plant pathology, 21(4), 541-554 (2020-02-23)
Proteases secreted by pathogens have been shown to be important virulence factors modifying plant immunity, and cysteine proteases have been demonstrated to participate in different pathosystems. However, the virulence functions of the cysteine proteases secreted by Phytophthora parasitica are poorly

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