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Merck

I4401

Sigma-Aldrich

Interferon-αA/D human

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture

Synonim(y):

IFN-αA/D

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About This Item

Numer MDL:
Kod UNSPSC:
12352202
NACRES:
NA.32

pochodzenie biologiczne

human

Poziom jakości

rekombinowane

expressed in E. coli

Próba

≥95%

Formularz

buffered aqueous solution

masa cząsteczkowa

19 kDa

opakowanie

pkg of 100K units

metody

cell culture | mammalian: suitable

zanieczyszczenia

endotoxin, tested

kolor

colorless

numer dostępu UniProt

Warunki transportu

dry ice

temp. przechowywania

−70°C

informacje o genach

human ... IFNA2(3440)

Zastosowanie

Interferon-αA/D human has been used:
  • for identification of coronavirus replicase inhibitors
  • in combination with ACH-806 [1-(4-pentyloxy-3-trifluoromethylphenyl)-3-(pyridine-3-carbonyl)thiourea] to study the effect on hepatitis C virus (HCV) replication
  • in combination with vitamin D to check reduction in HCV protein production in cell culture

Działania biochem./fizjol.

Interferon-αA/D (Bg/II), also known as Universal Type I Interferon, is an α interferon hybrid that crosses the species barrier. The Bg/II denotes the restriction enzyme site from which this hybrid is constructed from recombinant human interferons αA and αD. Interferon-αA/D has been proven to be active on many mammalian cells. It can substitute for human, monkey, mouse, bovine, rat, cat, dog, rabbit, sheep, goat, horse, pig or hamster type I interferon (α, β, ω and τ). Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes), and ruminant embryos (τ subtypes). This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.
This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Postać fizyczna

Solution in phosphate buffered saline containing 0.1% bovine serum albumin

Komentarz do analizy

The biological activity is determined in the cytopathic effect inhibition assay using MDBK cells with vesicular stomatitis virus (VSV). A cytopathic effect of 50% is produced with 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for human interferonαA.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Guido Wollmann et al.
Journal of virology, 89(13), 6711-6724 (2015-04-17)
High-grade tumors in the brain are among the deadliest of cancers. Here, we took a promising oncolytic virus, vesicular stomatitis virus (VSV), and tested the hypothesis that the neurotoxicity associated with the virus could be eliminated without blocking its oncolytic
Tobias Hertzig et al.
The Journal of general virology, 85(Pt 6), 1717-1725 (2004-05-29)
A previously unknown coronavirus (CoV) is the aetiological agent causing severe acute respiratory syndrome (SARS), for which an effective antiviral treatment is urgently needed. To enable the rapid and biosafe identification of coronavirus replicase inhibitors, we have generated a non-cytopathic
Sophie Rogée et al.
Antiviral research, 161, 1-9 (2018-10-28)
Rabies virus transmits from animals to humans and causes encephalitis. Every year more than 15 million people receive a post exposure prophylaxis (PEP) treatment that is highly effective in the prevention of rabies disease. However, when clinical symptoms appear, for
David L Wyles et al.
Antimicrobial agents and chemotherapy, 52(5), 1862-1864 (2008-03-12)
Rapid emergence of resistance to monotherapy with virus-specific inhibitors necessitates combination therapy. ACH-806 is a hepatitis C virus NS4A inhibitor with a novel mechanism of action and resistance pathway. This compound was synergistic with NS3 protease inhibitors and NS5B nucleoside
Hector Moreno et al.
Viruses, 13(7) (2021-08-11)
The New World (NW) mammarenavirus group includes several zoonotic highly pathogenic viruses, such as Junin (JUNV) or Machupo (MACV). Contrary to the Old World mammarenavirus group, these viruses are not able to completely suppress the innate immune response and trigger

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