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H7425
Anti-FLAG®-Peroxidase antibody produced in rabbit
IgG fraction of antiserum
Synonim(y):
Anti-FLAG-HRP Polyclonal Conjugate
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About This Item
Kod UNSPSC:
12352203
NACRES:
NA.43
Polecane produkty
Poziom jakości
opis
buffered aqueous solution
Formularz
lyophilized solid
metody
western blot: 1:2000-1:4000 using for detection of amino-terminal FLAG-BAP fusion protein in an E. coli crude cell lysate
Warunki transportu
dry ice
temp. przechowywania
−20°C
Opis ogólny
Anti-FLAG antibody is developed in rabbits using purified FLAG fusion protein as immunogen. Whole antiserum is purified using protein A immobilized on agarose to provide the IgG fraction of the antiserum and is conjugated to horseradish peroxidase.
ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. The antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins by immunoblotting. Specific staining is inhibited by the FLAG peptide (N-Asp-Tyr-Lys-Asp-AspAsp-Asp-Lys-C). Applications for the conjugate include Western blots and ELISA.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu2+ ions of the C-terminal FLAG peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG peptide is found in both rat and mouse Mg2+ dependent protein β-phosphatase, as well as in the human and bovine enzyme.
ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. The antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins by immunoblotting. Specific staining is inhibited by the FLAG peptide (N-Asp-Tyr-Lys-Asp-AspAsp-Asp-Lys-C). Applications for the conjugate include Western blots and ELISA.
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance, or poorly immunogenic proteins when protein specific antibodies are not available. Tagging with the FLAG peptide sequence may be done at the N-terminus, N-terminus preceded by a methionine residue, C-terminus, or at internal positions of the target protein. FLAG may also be placed in association with other tags. The small size of the FLAG tag or sequence and its high hydrophilicity tend to decrease the possibility of interference with the protein expression, proteolytic maturation, antigenicity, and function. The N-terminal FLAG peptide sequence contains a unique enterokinase cleavage site allowing it to be completely removed from the purified fusion proteins. Cleavage catalyzed by Cu2+ ions of the C-terminal FLAG peptide from a fusion protein has been reported. A sequence motif with five out of eight amino acid residues identical to the FLAG peptide is found in both rat and mouse Mg2+ dependent protein β-phosphatase, as well as in the human and bovine enzyme.
Immunogen
purified FLAG fusion protein
Zastosowanie
For immunoblotting, with 1:2000 – 1:4000 as an initial suggested dilution
Browse additional application references in our FLAG® Literature FLAG® Literature portal.
Browse additional application references in our FLAG® Literature FLAG® Literature portal.
Postać fizyczna
Lyophilized powder
Informacje prawne
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
For Research use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Chronic 3 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
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Lot/Batch Number
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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
R G Chubet et al.
BioTechniques, 20(1), 136-141 (1996-01-01)
The FLAG peptide, AspTyrLysAspAspAspAspLys, has been used as an epitope tag in a variety of cell types. The modification of the cytomegalovirus (CMV) promoter containing vector, pCMV5, to create two transient expression vectors designed for secretion and intracellular expression of
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