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Key Documents
F8888
Anti-Chicken IgY (IgG) (whole molecule)−FITC antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonim(y):
Rabbit Anti-Chicken IgY (IgG) (whole molecule)−Fluorescein isothiocyanate
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About This Item
Polecane produkty
pochodzenie biologiczne
rabbit
białko sprzężone
FITC conjugate
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
secondary antibodies
klon
polyclonal
Postać
buffered aqueous solution
warunki przechowywania
protect from light
metody
direct immunofluorescence: 1:320 using human peripheral blood lymphocytes
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:320 using human tonsil
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
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Opis ogólny
Birds, reptiles and amphibians express IgY antibodies that are functionally equivalent to IgG. Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for IgY of chicken. Rabbit Anti- Chicken IgY (IgG) is conjugated to fluorescein isothiocyanate (FITC). Free FITC is removed by gel filtration.
Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.
Specyficzność
Binds chicken IgY (IgG). Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody is specific for chicken IgY (IgG).
Immunogen
Purified chicken IgY (IgG)
chicken IgY (IgG)
Zastosowanie
Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody has been used in immunofluorescence.
Anti-Chicken IgY (IgG) (whole molecule)-FITC antibody may be used for immunofluorescence using human peripheral blood lymphocytes at a working antibody dilution of 1:320. For immunohistochemistry a working antibody dilution of 1:320 may be used. A dilution of 1:1000 was used for EP receptor staining of murine keratinocyte cell line 308 using primary antibodies generated in chicken. The antibody was also used for immune electron microscopy labeling of porcine nerve samples.
Działania biochem./fizjol.
Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus chicken antibodies are useful analytical tools for various research applications .
IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
nwg
Środki ochrony indywidualnej
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
Characterization of two recombinant HVT-IBD vaccines by VP2 insert detection and cell-mediated immunity after vaccination of specific pathogen-free chickens
Ingrao F, et al.
Avian Pathology : Journal of the W.V.P.A, 46(3), 289-299 (2017)
E J Thompson et al.
Neoplasia (New York, N.Y.), 3(5), 402-410 (2001-11-01)
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Serological evidence of West Nile virus, Usutu virus and Sindbis virus infection of birds in the UK.
Alan Buckley et al.
The Journal of general virology, 84(Pt 10), 2807-2817 (2003-09-19)
The introduction and rapid dispersal of the African flavivirus West Nile virus (WNV) throughout North America, and the high fatality rate due to encephalitis in birds, horses, other wildlife species and humans, has attracted major attention worldwide. Usutu virus, another
Stefan Magnusson et al.
Xenotransplantation, 12(1), 49-58 (2004-12-16)
The use of thin easily revascularized cutaneous nerve autografts, which has been the gold standard, or the alternative use of nerve allografts or artificial grafts for nerve reconstructing have all their pros and cons. Nerve xenotransplantation may offer a potential
G W Warr et al.
Immunology today, 16(8), 392-398 (1995-08-01)
IgY is the functional equivalent of IgG in birds, reptiles and amphibia, but many aspects of its biology are poorly understood. Recent studies have increased awareness of the genetics and functions of this molecule, and have revealed its position as
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