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F3174

Sigma-Aldrich

Fpg Protein from Escherichia coli

≥90% (SDS-PAGE), buffered aqueous glycerol solution, >20,000 units/mg protein, suitable for genomic analysis

Synonim(y):

DNA-(apurinic or apyrimidinic site)lyase MutM (APlyase MutM), Fapy-DNAglycosylase, Formamidopyrimidine-DNA glycosylase, Fpg Protein from Escherichia coli, Recombinant, Fapy DNA glycosylase, Formamidopyrimidine DNA glycosylase, MutM

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About This Item

Numer CAS:
78783-53-6
Numer EC enzymu:
3.2.2.23 (BRENDA, IUBMB)
Numer MDL:
MFCD02264758
Kod UNSPSC:
12352202
NACRES:
NA.32

pochodzenie biologiczne

Escherichia coli

Poziom jakości

200

rekombinowane

expressed in E. coli

Próba

≥90% (SDS-PAGE)

Postać

buffered aqueous glycerol solution

aktywność właściwa

>20,000 units/mg protein

masa cząsteczkowa

30.2 kDa (269 amino acids, predicted from the nucleotide sequence)

skład

protein, 0.1- 0.3 mg/mL Bradford

warunki przechowywania

(Tightly closed)

metody

nucleic acid detection: suitable

numer dostępu UniProt

P05523

Zastosowanie

genomic analysis

Warunki transportu

wet ice

temp. przechowywania

−20°C

informacje o genach

Escherichia coli CFT073 ... mutM(1038243)
Escherichia coli K12 ... mutM(946765)

Opis ogólny

Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found in Escherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.
Fpg contains two domains separated by a flexible hinge.

Research area: Cell signaling

Zastosowanie

Fpg Protein from Escherichia coli has been used for the assessment of DNA oxidative damage using comet assay.

Działania biochem./fizjol.

Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as an N-glycosylase and apurinic/apyrimidinic lyase.
Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action.
Fpg specifically acts on 3′- and 5′-phosphodiester bonds.

Definicja jednostki

One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine−mutated) in 10 min at 25 °C.

Postać fizyczna

Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

Kim Jantzen et al.
Mutagenesis, 27(6), 693-701 (2012-08-08)
Studies in mono-culture of cells have shown that diesel exhaust particles (DEPs) increase the production of reactive oxygen species (ROS) and oxidative stress-related damage to DNA. However, the level of particle-generated genotoxicity may depend on interplay between different cell types
Richard Meitern et al.
The Journal of experimental biology, 216(Pt 14), 2713-2721 (2013-04-13)
Oxidative stress (OS) is widely believed to be responsible for the generation of trade-offs in evolutionary ecology by means of constraining investment into a number of components of fitness. Yet, progress in understanding the true role of OS in ecology
Yan Qi et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(4), 1086-1091 (2012-01-06)
Base excision repair of genotoxic nucleobase lesions in the genome is critically dependent upon the ability of DNA glycosylases to locate rare sites of damage embedded in a vast excess of undamaged DNA, using only thermal energy to fuel the
Lotte Frigaard Mandsberg et al.
FEMS microbiology letters, 324(1), 28-37 (2011-11-19)
Prevention and correction of oxidative DNA lesions in Pseudomonas aeruginosa is ensured by the DNA oxidative repair system (GO). Single inactivation of mutT, mutY and mutM involved in GO led to elevated mutation rates (MRs) that correlated to increased development
Nikita A Kuznetsov et al.
Nucleic acids research, 40(15), 7384-7392 (2012-05-16)
Formamidopyrimidine-DNA glycosylase, Fpg protein from Escherichia coli, initiates base excision repair in DNA by removing a wide variety of oxidized lesions. In this study, we perform thermodynamic analysis of the multi-stage interaction of Fpg with specific DNA-substrates containing 7,8-dihydro-8-oxoguanosine (oxoG)
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