A9528

Amphotericin B solubilized

powder, suitable for cell culture, BioXtra

• Synonim(y): Amphotericin B from Streptomyces sp., Fungizone
• Wzór empiryczny (zapis Hilla): C₄₇H₇₃NO₁₇
• Numer CAS: 1397-89-3
• Masa cząsteczkowa: 924.08
• Beilstein: 78342
• Numer MDL: MFCD00877763
• Kod UNSPSC: 12352207
• eCl@ss: 34010400
• Identyfikator substancji w PubChem: 24891468
• NACRES: NA.76

Właściwości

• Nazwa produktu: Amphotericin B solubilized, powder, γ-irradiated, BioXtra, suitable for cell culture
• Poziom jakości: 300
• sterylność: γ-irradiated
• linia produktu: BioXtra
• Formularz: powder
• metody: cell culture | mammalian: suitable
• spektrum działania antybiotyku: fungi; yeast
• Tryb działania: cell membrane | interferes
• temp. przechowywania: 2-8°C
• ciąg SMILES: C[C@H]1O[C@@H](O[C@@H]2C[C@@H]3O[C@](O)(C[C@@H](O)C[C@@H](O)[C@H](O)CC[C@@H](O)C[C@@H](O)CC(=O)O[C@@H](C)[C@H](C)[C@H](O)[C@@H](C)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\2)C[C@H](O)[C@H]3C(O)=O)[C@@H](O)[C@@H](N)[C@@H]1O
• InChI: 1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
• Klucz InChI: APKFDSVGJQXUKY-INPOYWNPSA-N

Opis

Opis ogólny

Chemical structure: polyene

Zastosowanie

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Działania biochem./fizjol.

Amphotericin B binds to sterols, forming pores in the membrane, and causing small molecules to leak out. The product is most effective against fungi and yeast.

Opakowanie

Solubilized Amphotericin B is packaged in amber glass bottle.

Komponenty

After reconstitution with water, the product will be a suspension of Amphotericin B, containing deoxycholate as the solubilizing agent. The lyophilized formulation is ~45% Amphotericin B and 35% sodium deoxycholate in sodium phosphate and sodium chloride.

Przestroga

Amphotericin B remains active for 3 days in culture at 37°C. At -20°C, and with protection from air and light, the product remains active for 5 years.

Uwaga dotycząca przygotowania

Amphotericin B is soluble at 19.6-20.4 mg/ml in water, at natural pH without heating, giving a yellowish clear to slightly hazy solution.
Aqueous solutions cannot be sterile filtered due to poor solubility. If reconstituted in sterile water, there is no need to filter sterilize.

It is also soluble in DMSO (30-40 mg/mL) and in dimethylformamide (2-4 mg/mL).

Inne uwagi

Keep container tightly closed in a dry and well-ventilated place. Product is sensitive to light and moisture. Keep in a dry place.

The actual solid weight of the packaged product is calculated according to the UV purity.

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Tylko elementy zestawu

• Amphotericin B ~ 45 %
• Sodium deoxycholate ~ 35 %
• Sodium phosphate balance

Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H372
• Zwroty wskazujące środki ostrożności: P260 - P264 - P270 - P314 - P501
• Klasyfikacja zagrożeń: STOT RE 1
• Kod klasy składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Gloves