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F2772
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody produced in goat
flow cytometry grade, affinity isolated antibody, buffered aqueous solution
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About This Item
Polecane produkty
pochodzenie biologiczne
goat
Poziom jakości
białko sprzężone
FITC conjugate
forma przeciwciała
affinity isolated antibody
rodzaj przeciwciała
secondary antibodies
klasa czystości
flow cytometry grade
klon
polyclonal
Formularz
buffered aqueous solution
warunki przechowywania
protect from light
metody
flow cytometry: 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
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Opis ogólny
IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Mouse IgG is a plasma B cell derived antibody isotype defined by its heavy chain. IgG is the most abundant antibody isotype found in mouse serum. IgG crosses the placental barrier, is a complement activator and binds to the Fc-receptors on phagocytic cells. The level of IgG may vary with the status of disease or infection.
Anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. The F(ab′)2 antibodies isolated from goat antiserum by affinity chromatography, react specifically with mouse IgG Fc; does not bind other mouse Igs. No cross reaction is observed with mouse Fab fragment. The antibody shows minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.
Anti-mouse IgG is conjugated to Fluorescein Isothiocyanate (FITC), Isomer I. The F(ab′)2 antibodies isolated from goat antiserum by affinity chromatography, react specifically with mouse IgG Fc; does not bind other mouse Igs. No cross reaction is observed with mouse Fab fragment. The antibody shows minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.
Specyficzność
Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment−FITC antibody isolated from goat antiserum by affinity chromatography reacts specifically with mouse Fc fragment. No cross reaction is observed with mouse Fab fragment. The antibodies are adsorbed to assure minimal cross reaction with human, horse and bovine proteins on tissue or cell preparations.
Immunogen
Purified Fc fragments of mouse IgG
Zastosowanie
Anti-Mouse IgG (Fc specific) F(ab′)2 fragment-FITC antibody produced in goat has been used in :
- flow cytometry for labelling of human peripheral blood lymphocytes (dilution of 1:100) and label human polymorphonuclear cells (PMNs) (dilution of 1:50).
- immunocytochemistry using human embryonic stem cells
- immunohistochemistry at a working dilution of 1:160.
Goat polyclonal anti-Mouse IgG (Fc specific) F(ab′)2 fragment-FITC antibody may be used as a reagent in flow cytometry, immunohistology and immunocytology offering sensitive and specific activity to mouse IgG and no cross reactivity with human, horse or bovine immunoglobulins.
Immunohistology: A minimum dilution of 1:160 is determined by indirect immunofluorescence on formalinfixed, and paraffin-embedded human tonsil using mouse monoclonal anti-human IgG as the primary antibody.
Immunohistology: A minimum dilution of 1:160 is determined by indirect immunofluorescence on formalinfixed, and paraffin-embedded human tonsil using mouse monoclonal anti-human IgG as the primary antibody.
Działania biochem./fizjol.
immunoglobulin G (IgG) antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. IgG antibody participates in complement fixation and opsonization.
Inne uwagi
Antibody adsorbed with bovine, equine and human serum proteins.
Postać fizyczna
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Uwaga dotycząca przygotowania
Adsorbed to reduce background with bovine, horse or human samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Useful when trying to avoid background staining due to the presence of Fc receptors.
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Środki ochrony indywidualnej
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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