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DIF001
3T3-L1 DIFFERENTIATION KIT
Sufficient for 100 mL differentiation medium
Synonim(y):
3T3-L1 Differentiation Kit
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About This Item
Kod UNSPSC:
12352207
NACRES:
NA.75
Polecane produkty
Postać
solution
zastosowanie
100 mL differentiation medium
metody
cell culture | mammalian: suitable
Warunki transportu
wet ice
temp. przechowywania
−20°C
Opis ogólny
3T3-L1 cells are derived from mouse 3T3 cells and provide a widely-used fundamental model in the study of adipose physiology and metabolic diseases. They exhibit a fibroblast-like morphology before differentiation but become rounded and accumulate lipid droplets several days after the initiation of differentiation. The accumulated lipid droplets can be visualized by light microscopy. The in vitro differentiated 3T3-L1 adipocytes result in characteristics similar to tissue-derived adipocytes and have been commonly used to study adipogenesis, lipolysis, and metabolic dysfunctions. The 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes.
Zastosowanie
3T3-L1 DIFFERENTIATION KIT has been used in:
- Differentiation of 3T3-L1 preadipocytes to adipocytes.
- Study of obesity, adipogenesis, lipolysis and lipid metabolism
- Differentiation of 3T3-L1 preadipocytes to adipocytes
- Study of obesity, adipogenesis, lipolysis and lipid metabolism.
Komponenty
Insulin (1.5 mg/ml) 0.6 ml (Catalog Number DIF001A, Green Cap)
Differentiation Cocktail, 1000x (lyophilized) 1 vl (Catalog Number DIF001B, Yellow Cap)
DMSO (anhydrous) 0.5 ml (Catalog Number DIF001C, Blue Cap)
Differentiation Cocktail, 1000x (lyophilized) 1 vl (Catalog Number DIF001B, Yellow Cap)
DMSO (anhydrous) 0.5 ml (Catalog Number DIF001C, Blue Cap)
Przechowywanie i stabilność
Store the kit at −20 °C, protected from light.
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Repr. 1B
Kod klasy składowania
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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