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Key Documents
CS1070
Transglutaminase Assay Kit
sufficient for assays in two 96-well plates
Synonim(y):
TGases Assay Kit
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About This Item
Kod UNSPSC:
12161503
NACRES:
NA.28
Polecane produkty
Poziom jakości
Warunki transportu
dry ice
temp. przechowywania
−20°C
informacje o genach
human ... TGM1(7051) , TGM2(7052) , TGM3(7053) , TGM4(7047) , TGM5(9333) , TGM6(343641) , TGM7(116179)
Opis ogólny
The Transglutaminase Assay Kit is designed for the detection of transglutaminase activity in biological samples. The kit can also be used for screening of transglutaminase inhibitors/activators.
Zastosowanie
The assay is based on transglutaminase catalysis of a covalent bond formation between the free amine group of Poly L-Lysine, which is covalently attached to the plate surface, and the γ-carboxamide group of biotin-TVQQEL-OH substrate present in the assay buffer. This reaction immobilizes biotin to the plate surface. The amount of immobilized biotin is proportional to the amount of active transglutaminase in the sample. The amount of immobilized biotin is determined using streptavidin-peroxidase and TMB substrate.
Działania biochem./fizjol.
Transglutaminases (TGases) are a widely distributed and unique group of calcium dependent enzymes that catalyze the post-translational modification of proteins by the formation of isopeptide bonds. This occurs either through protein cross-linking via formation of γ-glutamyl-ε-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The cross-linked products, often of high molecular mass, are highly resistant to mechanical challenge and proteolytic degradation, and their accumulation is found in a number of tissues and processes, where such properties are important including skin, hair, blood clotting, and wound healing. Deregulation of the enzyme activity contributes to a number of human diseases.
Inne uwagi
The detection limit is 0.003 mU/assay or 0.03mU/ml of Transglutaminase.
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Elementy zestawu są też dostępne oddzielnie
Numer produktu
Opis
Karta charakterystyki
- T5398Transglutaminase from guinea pig liver, lyophilized powder, ≥1.5 units/mg protein 2 eaKarta charakterystyki
- T04403,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, peroxidase substrateKarta charakterystyki
- S5512Streptavidin−Peroxidase from Streptomyces avidinii, lyophilized powder 100 μgKarta charakterystyki
Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2
Kod klasy składowania
8A - Combustible corrosive hazardous materials
Certyfikaty analizy (CoA)
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Klienci oglądali również te produkty
Mechanism of action of guinea pig liver transglutaminase. VII. Chemical and stereochemical aspects of substrate binding and catalysis.
S I Chung et al.
The Journal of biological chemistry, 245(23), 6424-6435 (1970-12-10)
Bovine aortic endothelial cell transglutaminase. Enzyme characterization and regulation of activity.
G Korner et al.
The Biochemical journal, 262(2), 633-641 (1989-09-01)
Bovine aortic endothelial cells contain Ca2+-dependent tissue-type transglutaminase. Its activity in these cells was high, with apparent Km and Vmax. values with respect to putrescine of 0.203 mM and 18.5 nmol/min per mg of protein, and its activity was inhibited
Transglutaminases and their substrates.
Francesco Facchiano et al.
Progress in experimental tumor research, 38, 37-57 (2005-03-05)
Seong-Gon Kim et al.
Oncology reports, 25(6), 1597-1602 (2011-03-23)
Resistance to chemotherapy is very important in the prognosis of tumors. Transglutaminase-2 (TG-2) mediated chemotherapy resistance has been widely reported. The objective of this study was to demonstrate the effect of 4-hexylresorcinol (4-HR) on TG-2 activity in nasopharyngeal squamous cell
Binh V Le et al.
The Journal of biological chemistry, 288(7), 4844-4853 (2013-01-05)
Male Anopheles mosquitoes coagulate their seminal fluids via cross-linking of a substrate, called Plugin, by the seminal transglutaminase AgTG3. Formation of the "mating plug" by cross-linking Plugin is necessary for efficient sperm storage by females. AgTG3 has a similar degree
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