CS0004

Nuclease micrococcal from Staphylococcus aureus

With reaction buffer and BSA solution

• Synonim(y): Nuclease micrococcal from Staphylococcus aureus, Endonuclease micrococcal, MNase, Micrococcal endonuclease
• Numer CAS: 9013-53-0
• Numer EC enzymu: 3.1.31.1 (BRENDA, IUBMB)
• Numer MDL: MFCD00131731
• Kod UNSPSC: 12352202
• NACRES: NA.77

Właściwości

• pochodzenie biologiczne: Staphylococcus aureus
• Postać: liquid
• Warunki transportu: wet ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Nuclease Micrococcal (MNase) is the extracellular nuclease of Staphylococcus aureus. The enzyme is an endo-exonuclease .This Endo-exonuclease preferentially digests single-stranded nucleic acids. Thus, nuclease micrococcal activity results in the production of mononucleotides and oligonucleotides with terminal 3′-phosphates. Nuclease Micrococcal is also active against double-stranded DNA and RNA and all sequences will be ultimately cleaved.
Micrococcal Nuclease can be used for the digestion of chromatin to identify the location of nucleosomes

Zastosowanie

• Chromatin Immuno-Percipitation (ChIP) analysis
• Nucleosome preparation
• Removal of nucleic acids present in protein preparation
• Reduction of viscosity of cell lysates during non-mechanical cell lysis preparation
• RNA sequencing and transcriptomic profiling

Działania biochem./fizjol.

Hydrolyzes 5′-phosphodiester bonds of DNA.

Inne uwagi

Note: One μmolar unit = approx. 85 A₂₆₀ units, where an A₂₆₀ unit is equivalent to a ΔA₂₆₀ of 1.0 in 30 min at pH 8.8 at 37 °C in a 3.55 mL reaction volume. Light path = 1 cm.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids