CRISPR18V

Lenti CRISPR Universal Non-Target Control#1 Transduction Particles (LV04 vector)

• Kod UNSPSC: 41106609
• NACRES: NA.51

Właściwości

• Postać: liquid
• Poziom jakości: 200
• opakowanie: vial of 200 μL
• stężenie: (1x10⁶ TU/ml (via p24 titering assay))
• Zastosowanie: CRISPR
• Warunki transportu: dry ice
• temp. przechowywania: −70°C

Opis

Opis ogólny

The Lenti CRISPR Non-Target Control#1 Transduction Particles (LV04 vector) include a gRNA sequence that does not target known human, mouse and rat genes. The non-target control#1 transduction particles are useful as a negative control in experiments using CRISPR lentiviral clones and for cell types that are not amenable to transfection. The Lenti CRISPR Non-Target Contro#1 usesl a dual-component system consisting of U6-driven guide RNA (non-target#1) and hPGK-driven Puromycin resistance cassette and Blue Fluorescence Protein for fluorophorescence. This control requires Cas9 be delivered by a separate vector.

Lentiviral-based particles permit efficient infection and integration of the construct into differentiated and non-dividing cells, such as neurons and dendritic cells, overcoming low transfection and integration difficulties when using these cell lines. Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging and envelope plasmids.

Zastosowanie

Functional Genomics/Screening/Target Validation

Inne uwagi

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells, respectively.

Informacje prawne

CRISPR Use License Agreement

Lentiviral, WPRE, and Evrogen Licenses

This page may contain text that has been machine translated.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable