NCI-H358
95111733, human lung (bronchoalveolar), Not specified
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About This Item
Kod UNSPSC:
41106514
Polecane produkty
product name
NCI-H358, 95111733
pochodzenie biologiczne
human lung (bronchoalveolar)
tryb wzrostu
Adherent
kariotyp
Not specified
morfologia
Not specified
produkty
Not specified
receptory
Not specified
metody
cell culture | mammalian: suitable
powiązane choroby
cancer
Warunki transportu
dry ice
temp. przechowywania
−196°C
Pochodzenie linii komórkowej
Human Caucasian bronchioalveolar carcinoma
Opis linii komórkowej
NCI-H358 was isolated from a primary bronchioalveolar carcinoma of the lung from a Caucasian male taken prior to treatment. Ultrastructural studies of this non-small cell carcinoma of the lung (NSCLC) demonstrated the presence of granules characteristic of Clara cells. NCI-H358 do not express UDP-glucuronosyltransferases, but do express glutathione-S-transferase and phenol sulphotransferase. Expression of SP-A protein and RNA, the major surfactant-associated protein was detected. SP-B and SP-C RNA was not expressed. A complete homozygous deletion of the p53 gene and therefore a lack of p53 protein has been reported. A colony forming efficiency of 0.83% in soft agarose, and growth in serum-free media has been reported. The cells are tumourigenic in athymic nude mice, and exhibit a doubling time of 38 hours in RPMI 1640 medium. Since these cells are proficient in oxidation of xenobiotics but deficient in their conjugation with glucuronic acid they present a tool for analysing the role of glucuronic acid conjugation in the inactivation of chemicals in intact cells.
Zastosowanie
Test system for evaluating cytotoxicity and genotoxicity of chemicals to human lung
Profil DNA
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12,13
D5S818: 10,12
D7S820: 10,11
THO1: 6
TPOX: 8,9
vWA: 17
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12,13
D5S818: 10,12
D7S820: 10,11
THO1: 6
TPOX: 8,9
vWA: 17
pożywka hodowlana
RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).
Rutyna subkultury
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Inne uwagi
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