3T3(+3)
89022402, mouse unknown/unspecified, Fibroblast
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About This Item
Kod UNSPSC:
41106514
Polecane produkty
product name
3T3(+3), 89022402
pochodzenie biologiczne
mouse unknown/unspecified
tryb wzrostu
Adherent
kariotyp
Not specified
morfologia
Fibroblast
produkty
Not specified
receptory
Not specified
metody
cell culture | mammalian: suitable
Warunki transportu
dry ice
temp. przechowywania
−196°C
Pochodzenie linii komórkowej
Mouse
Opis linii komórkowej
Please note: Available stocks of this cell line are not contact-inhibited. Contact inhibition is the natural process of arresting cell growth when cells come into contact with each other. It is not possible to be sure how the loss of contact inhibition may affect other characteristics of the cell line. This cell line catalog number continues to be a popular choice.
The key consideration is whether the characteristic of contact inhibition is required for cell line work. For example, it is required when testing the affects of expression of potential oncogenes where the induction of the loss of contact inhibition is being measured, whereas it is of less significance when using the cell line to express recombinant proteins for production and purification.
The key consideration is whether the characteristic of contact inhibition is required for cell line work. For example, it is required when testing the affects of expression of potential oncogenes where the induction of the loss of contact inhibition is being measured, whereas it is of less significance when using the cell line to express recombinant proteins for production and purification.
pożywka hodowlana
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Rutyna subkultury
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Inne uwagi
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