NCTC clone 929

88102702, mouse connective tissue, Fibroblast

• Synonim(y): Earles′s Cells, L Cells, L-929 Cells, NCTC 929 Cells, NCTC-929L Cells
• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: NCTC clone 929, 88102702
• pochodzenie biologiczne: mouse connective tissue
• tryb wzrostu: Adherent
• kariotyp: 2n = 40
• morfologia: Fibroblast
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Mouse C3H/An connective tissue

Opis linii komórkowej

From parental strain L which was derived from normal subcutaneous areolar and adipose tissue of 100 day old male C3H/An. Used in development of replicate culture techniques, chemically defined media, fluid suspension culture methods and virus assays. Susceptible to pseudorabies virus and vesicular stomatitis (Indiana strain) virus.

Zastosowanie

Replication, biochemical, neoplastic transformation, nutritional, metabolic enzyme, morphological, chromosome, immunologic, radiation, toxicologic and virus studies

pożywka hodowlana

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Freeze in 10% glycerol/90% FBS. After resuscitation cells may take up to 4 days to establish growth.

Inne uwagi

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