KT12 human cell line

88012503, IHW Number: 9073

• Synonim(y): LKT12
• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: human blood
• pochodzenie etniczne: Japanese
• płeć: male
• tryb wzrostu: Suspension
• kariotyp: Not specified
• morfologia: Lymphoblastoid
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice

Opis

Opis ogólny

Consanguineous: No

Opis linii komórkowej

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Profil DNA

A: 2402 31012
Cw: 0401 1202
B: 3501 5101 (!)52
HLA DRB1: 09
HLA DQB1: 03
HLA DPB1: 0402 0501

Profil serologiczny

HLA A: 24 31
HLA A ief: 24.1 31
HLA C: 4
HLA B: 35 51
HLA B ief: 35.3 51
Bw4/bw6: 4 6
HLA Dw: 23
HLA DR: 9
HLA DR53: 53
HLA DQ: 9
HLA DP: 5

pożywka hodowlana

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rutyna subkultury

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Inne uwagi

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Oświadczenie o zrzeczeniu się odpowiedzialności

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

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